葡萄糖转运蛋白9论文SLC2A9基因第八外显子137AG位点多态性与糖尿病合并高尿酸血症的关系.doc

葡萄糖转运蛋白9论文：SLC2A9基因第八外显子137A/G位点多态性与糖尿病合并高尿酸血症的关系 【中文摘要】探讨山东省青岛市汉族人SLC2A9基因第八外显子137A/G(rs3733591)位点单核苷酸多态性与糖尿病合并高尿酸血症的关系。方法：应用序列特异性引物-聚合酶链式反应技术(PCR)扩增SLC2A9基因第八外显子,取PCR产物经限制性内切酶BstUI酶切,对部分PCR产物进行测序,检测414例在青医附院就诊的患者SLC2A9基因第八外显子137A/G单核苷酸多态性位点的等位基因频率和基因型频率的分布情况。结果：SLC2A9基因第八外显子137A/G多态性位点A等位基因频率在对照组、高尿酸血症组、糖尿病组、糖尿病合并高尿酸血症组分别为68.1%、58.6%、63.6%、58.2%,G等位基因频率分别为31.9%、41.4%、36.4%、41.8%,A、G的等位基因频率在对照组与高尿酸血症组、对照组与糖尿病合并高尿酸血症组间的差别有统计学意义(P0.05),在其余各组间的差别无统计学意义(P0.05)。AA基因型频率在对照组、高尿酸血症组、糖尿病组、糖尿病合并高尿酸血症组分别为45.1%、36.2%、41.7%、30.8%,AG基因型频率分别为46.1%、44.8%、43.7%、54.8%,GG基因型频率分别为8.8%、19.0%、14.6%、14.4%,三种基因型频率在各组之间的差异无统计学意义(P0.05)。结论：山东省青岛市汉族人SLC2A9基因第八外显子137A/G位点单核苷酸多态性与糖尿病合并高尿酸血症有相关性,携带等位基因G的个体发生糖尿病合并高尿酸血症的风险可能高于携带等位基因A的个体。 【英文摘要】:To investigate the single-nucleotide polymorphism(SNP) at position 137A/G of exon 8 of SLC2A9 gene for the association with diabetes mellitus and hyperuricemia in the Chinese Han population in Qingdao City of Shandong province.Methods:A segment of exon 8 of SLC2A9 gene was amplified by sequence specific primers and polymerase chain reaction (PCR) method, then all PCR products were digested by restriction enzyme BstUI and a part of PCR products were submitted to DNA sequence analysis, and we detected the differences of the allele frequency and genotype frequency of SLC2A9 gene at position 137A/G in 414 patients in affiliated hospital of medical college of Qingdao university in Shandong province.Results:A allele frequency of 137A/G polymorphism of exon 8 of SLC2A9 gene in control group is 68.1%, in hyperuricemia group is 58.6%, in diabetes mellitus group is 63.6%, and in diabetes mellitus and hyperuricemia group is 58.2%, while G allele frequency in control group is 31.9%, in hyperuricemia group is 41.4%, in diabetes mellitus group is 36.4%, and in diabetes mellitus and hyperuricemia group is 41.8%. The difference of allele frequency between control group and hyperuricemia group had statistical significance(P0.05), the difference of allele frequency betwee