防臭鞋垫的抗菌性能评价.doc

防臭鞋垫的抗菌性能评价 董海燕deodorant insoles DONG Hai-yan ( Fujian Provincial Fiber Inspection Institute 350026, China) [Abstract] Objective To evaluate the antibacterial property of deodorant insoles. Methods Agar diffusion plate method was used. Bacteriostasis bandwidth was calculated and the antiba-cterial property was evaluated on the level of qualitation. Shake-flask method was used at 24℃ and 150r/min rotational speed of conatant temperature oscillator. The deodorant insoles and bacteria acted for 18h in PBS liquid. The bacteriostasis rate was calculated and the antibacterial property was evaluated through colony counting method on the level of quantitat-ion Results Bacteriostasis bandwidth was ＞1mm，and Bacteriostasis band Bacteriostasis rate was 90.5% and 96.7% to coliform and staphlococcus，respectively. Conclustion The deodorant insoles has good antibacterial property [Key words] Deodorant insoles; Antibacterial property; Bacteriostasis bandwidth; Bacteriostasis rate 防臭鞋垫具有抗菌、除臭等多项医疗保健功效。一般采用夹层中药或特殊天然材料，经独特配方精制而成对脚气、脚痒等有特效。防臭鞋垫主要目的是在穿着状态下抑制以汗和污物为营养源的微生物的繁殖，保持卫生状态，其作为一种功能性鞋垫，目前已经投入市场并被消费者认可[1]。本文采用琼脂平皿扩散法和振荡法，从微生物学角度对防臭鞋垫的抗菌性能进行定性和定量评价。 1　材料与方法 1.1 材料 1.1.1 实验材料：防臭鞋垫，，，，1.1.4 仪器和设备：ZSD-1270型生化培养箱，上海有限公司，，上海有限公司，，，，，，，8 CFU/mL。 1.2.2 试样制备：从鞋垫上选取有代表性的试样，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，，b×100%，，，，，，5 3.25×103 97.4% 金黄色葡萄球菌 1.11×105 9.21×103 91.7% 注：n为实验重复次数 3 讨论 防臭鞋垫属于抗菌纺织品[4]的一种。本身具有良好的抗菌防臭性能，，，，，，，，，，，，，，[1] 车民. 抗菌防臭纺织品性能评价[J]. 合成技术及应用,2001,16(3):33,43,53. [2] GB/T 20944.1-2007 纺织品抗菌性能的评价 第一部分：琼脂平皿扩散法[S]. [3] GB/T 20944.1-2007 纺织品抗菌性能的评价 第三部分：振荡法[S]. [4] 王俊起,王有斌. 纺织品抗菌功能方法研究[J]. 中国卫生工程学,2003,2(3): 129. [5] 倪语星. 微生物学和微生物学检验试验指导[M]. 北京：人民卫生出版社,2001: 25. 1