雌激素污染对水体微生物产甲烷功能的影响.doc

雌激素污染对水体微生物产甲烷功能的影响( 赵颖1，阮爱东1,#，刘忱潇1，谢显传2 （1.河海大学水文水资源与水利工程科学国家重点实验室，中国，南京，210098； 2.南京大学水科学研究中心，中国，南京，210093 ） 摘要：针对目前水体环境雌激素的污染形势严峻，且其对水体功能性微生物群落影响规律及机理不明等问题，选择典型天然雌激素——17β-雌二醇(E2)为主要研究对象，构建实验室厌氧水体微生态系统，分析E2污染对厌氧水体微生物活性和产甲烷功能的影响规律。结果表明，在厌氧条件下，E2污染对水体微生物群体的活性存在显著的抑制效应，对水体甲烷的产生速率亦存在规律性的影响。当水体E2浓度≤0.5ng/L时，对水体微生物的产甲烷活性存在促进作用。当E2浓度≥1.0ng/L时，E2污染对水体微生物的产甲烷活性存在明显的抑制效应，且随着E2浓度的升高抑制作用增强。因此，厌氧条件下，微量E2污染会影响水体微生物活性和产甲烷效应的变化，从而对水体自净功能和甲烷释放产生影响。 关键词：17β-雌二醇；产甲烷菌；厌氧微生物活性；产甲烷功能 Impact of trace estrogens contamination on the anaerobic microbial and methanogenic activity Ying Zhao1， Aidong Ruan1，#，Chenxiao Liu1， Xianchuan Xie2 State key laboratory of hydrology-water resources and hydraulic engineering, Hohai University, Nanjing, P.R. China; 2.Center for Hydrosciences Research, Nanjing University, Nanjing, P. R. China) Abstract: The environmental estrogens pollution in aqueous systems is seriously in recent years. And the underlying mechanism on how they affect the functional microorganism groups in water body has not been known. In this study, the typical estrogen——17β-estradiol (E2) was chosen as the study object. Microecological systems in anaerobic pore water constructed in laboratory were used to analyze the impacts of E2 on microbial activities and methanogenic function. The results showed that E2 had an obvious inhibition effect on microbial activities under anaerobic condition while it also had a regularity effect on methane production rate of water. When the concentrations were ≤0.5ng/L, E2 played an enhancing role in methanogenic activity. But it inhibited methanogenesis when the concentrations exceeded 1.0ng/L, and the inhibition enhanced with the increase of concentrations. This research indicated that E2 in low concentrations can affect microbial activities and methanogenic effect in anaerobic water body, therefore influence the self-purification function of water bodies and emission of methane. Keywords: 17β-estradiol; methanogen; anaerobic microbial activity; m