雷公藤甲素对哮喘小鼠气道重塑及转化生长因子β1表达的影响.doc

雷公藤甲素对哮喘小鼠气道重塑及转化生长因子-β1表达的影响 作者：吕志强1， 陈 茗1， 江山平1*， 谭艳芳1， 张 蔚1， 梁瑞韵1， 李海刚2 作者单位：(中山大学附属第二医院 1. 呼吸内科; 2. 病理科， 广东 广州 510120) 【摘要】 【目的】 探讨雷公藤甲素对支气管哮喘(简称哮喘)小鼠气道重塑的影响。【方法】 40只雌性SPF级BABL/c小鼠，随机数字表法分为4组，每组10只。A组为生理盐水对照组;B组为卵蛋白(OVA)哮喘组;C组为雷公藤甲素治疗组;D组为地塞米松治疗组。在末次激发24 h后所有小鼠取左肺组织行苏木精-伊红(HE)染色，过碘酸-雪夫(PAS)染色，Masson三色染色以及抗转化生长因子 1(TGF- 1)抗体免疫组化染色。测定支气管管壁厚度(WAt/Pbm)，支气管平滑肌厚度(WAm/Pbm)。取右肺组织行RT-PCR检测TGF- 1 mRNA表达。【结果】 B组小鼠支气管管壁厚度、杯状细胞百分数、气道平滑肌厚度、胶原纤维面积、TGF- 1的转录和表达水平均显著高于A组(P 0.01)。C组小鼠上述各指标依次均显著低于B组(P 0.05)，D组小鼠上述指标显著低于B组(P 0.05)。但C组和D组上述各指标间差异无统计学意义(P 0.05)。肺组织的TGF- 1的蛋白表达水平与支气管的管壁厚度，平滑肌厚度，杯状细胞比，胶原纤维含量成正相关(相关系数分别为0.755、0.815、0.898、0.837;P 0.01)。 【结论】 雷公藤甲素可抑制BABL/c哮喘小鼠气道重塑的发生，其机制有可能是通过抑制TGF- 1的表达而实现的。 【关键词】 哮喘; 气道重塑; 小鼠; 雷公藤甲素 Effects of Triptolide on Airway Remodeling and TGF- 1 Expression in Asthmatic Mice LV Zhi-qiang1， CHEN Ming1， JIANG Shan-ping1*， TAN Yan-fang1， ZHANG Wei1， LIANG Rui-yun1， LI Hai-gang2 (1. Department of Respiratory Medicine; 2. Department of Pathology， The Second Affiliated Hospital， Sun Yat-sen University， Guangzhou 510120， China) Abstract： 【Objective】 To investigate the effect of triptolide on airway remodeling in asthmatic mice. 【Methods】 Forty female BABL/c mice were randomly divided into four groups with 10 mice in each group. ①Group A(control group)：mice were treated with saline; Group B(asthmatic group)：mice were sensitized and challenged with OVA; Group C(triptolide group)：mice were sensitized and challenged as asthmatic group， and treated with 40 g triptolide before challenged; Group D(dexamethasone group)：mice were sensitized and challenged as above，and were given 2 mg dexamethasone by intraperitoneal injection before challenged. All mice were killed 24 h after the final OVA challenge. The left lung was isolated for pathological examination. Lung sections were stained with hematoxylin and eosin (HE)， PAS， Masson?蒺s trichrome and immunohistochemical staining for TGF- 1 were performed.The thickness of bronchial airway (WAt/Pbm)， the