非小细胞肺癌患者血清及癌组织中P185neu的定量检测.doc

非小细胞肺癌患者血清及癌组织中P185neu的定量检测 　　作者：李新举，梁景仁，关正，袁育康，张智慧 作者单位：西安交通大学医学院第一附属医院胸外科，陕西西安 710061;西安交通大学医学院免疫学与病原生物学系，陕西西安 710061;四川省肿瘤医院肿瘤内科，四川成都 610041) 　　【摘要】 目的 探讨P185neu蛋白的定量水平与非小细胞肺癌(NSCLC)患者的临床生物学行为及预后的关系。方法 采用双抗体夹心ELISA法定量检测了37例NSCLC患者及17例良性肺疾病和健康对照者血清和组织中P185neu 的蛋白水平。结果 37例NSCLC患者血清和癌组织中P185neu 的蛋白水平明显升高(P0.01)，且P185neu 蛋白水平与NSCLC细胞分化程度、TNM分期及淋巴结转移呈显著正相关(P0.05)。另外，NSCLC患者术前血清P185neu 的蛋白水平明显高于术后10天的血清水平(P0.01)。结论 P185neu 的蛋白水平与NSCLC的发生、发展和转移密切相关;血清P185neu蛋白水平可作为评估NSCLC进展、复发和预后的有效指标。 　　【关键词】 肺癌;P185neu 蛋白;双抗体夹心ELISA法 　　Determination of P185neu level in serum and　tumour tissue of nonsmall cell lung cancer 　　Li Xinju, Liang Jingren, Guan Zheng, Yuan Yukang, Zhang Zhihui 　　(Department of Thoracic Surgery, the First Affiliated Hospital, Medical School of　Xian Jiaotong University, Xian 710061; Department of Immunology and　Microbiology, Medical School of Xian Jiaotong University, Xian 710061;Department of Oncology, Oncological Hospital of Sichuan Province, Chengdu 610041, China 　　ABSTRACT: Objective To investigate the relation between the level of P185neu in serum and tumour tissue and clinical biological behaviour of nonsmall cell lung cancer (NSCLC). Methods By ELISA method, 37 specimens of sera and tumour tissues of nonsmall cell lung cancer were examined, and 17 cases of adjacent nonneoplastic tissues and sera were examined for the level of P185neu protein. Results Of the 37 NSCLC cases, the level of P185neu in sera and tumour tissues was higher (P0.01). The level of P185neu was positively related to cell differentiation, TNM stages, and lymph node metastasis (P0.05). The preoperative serum level of P185neu was significantly higher than the postoperative serum level (P0.01). Conclusion P185neu may play an important role in tumor progression and malignant potential of nonsmall cell lung cancer, and the level of serum P185neu could be of prognostic significance. 　　KEY WORDS: lung cancer; P185neu ; BAELISA 　　近年来，随着分子生物学和遗传学研究的深入，