腺纤维腺瘤与乳腺浸润性导管癌血清代谢组学比较研究.doc

基于核磁共振1H谱的乳腺纤维腺瘤与乳腺浸润性导管癌血清代谢组学比较研究 刘影1,3，冀天星2，李江超1，王丽京1, 杨永霞 3,1﹡ 2 广州医科大学第二附属医院 检验科，广州 510260 3 广东药学院基础学院物理与电子学教研室510006 基金项目：国家自然科学基金（No.、广东省自然科学基金（No. S2011010002512） Supported by the General Programs of National Natural Science Foundation of China and National Natural Science Foundation of Guangdong province (S2011010002512) 作者简介：刘影，女，山东临沂人，硕士研究生，Email：liuying151633@163.com. 通讯作者：杨永霞，女，山东泰安人，博士，副教授，硕士生导师，Tel: 020 Email: sheepling-2004@163.com 。 [摘要] 目的 分析乳腺纤维腺瘤病人与乳腺浸润性导管癌病人在血清代谢组学上的差异，探讨术前通过代谢特征来辨别良恶性肿瘤的可能性，从而为临床诊断提供代谢依据。方法 收集经临床确诊的乳腺纤维腺瘤和乳腺浸润性导管癌病人血清各8例，通过核磁共振1H NMR技术检测其血清代谢组特征，分别采用主成分分析(principle component analysis，PCA)和正交最小偏二乘—判别法 (orthogonal signal correction partial least squares discrimination analysis ，OPLS-DA)分析纤维腺瘤与浸润性导管癌两者之间的代谢差异。结果 PCA和OPLS-DA [关键词] 核磁共振；乳腺纤维腺瘤； 浸润性导管癌；代谢组学 The 1H NMR-based serum metabonomics study of breast fibroadenoma and breast invasive ductal carcinoma patients LIU Ying1, 3, JI Tian-xing2, LI Jiang-chao1, WANG Li-jing1, YANG Yong-xia3 1 * 1 Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China 2 Department of clinical laboratory, the second affiliated hospital of Guangzhou Medical University, Guangzhou, 510260, Guangdong, China 3 Department of Physics and Electronic Teaching, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China [Abstract] Objective To research the serum metabonomic difference between breast fibroadenoma patients and breast invasive ductal carcinoma patients based on 1H NMR spectroscopy and to offer reliable biochemical information for clinical diagnosis. Methods The clinical diagnosed 8 breast fibroadenoma patients and 8 breast invasive ductal carcinoma patients were recruited and their sera spectra were collected in the method of 1H NMR. The data were processed using Simca-p software and analyzed with principle component analysis(PCA) and orthogonal signal correction partial