产红色素果蔬内生菌菌种鉴定【DOC精选】.docVIP

安徽农业科学。Journal 0fAnhui A鲥．Sci．2008，36(25 J：10756—10757 责任编辑王淼责任校对傅真治 产红色素果蔬内生菌菌种鉴定 王红庆(信阳农业高等专科学校，河南信阳464000) 摘要[目的]鉴定来自果蔬中的产红色素的内生茵菌株。[方法]以来源于果蔬中的内生茵株为试验材料，对其进行生理生化特性分析 及形态学和分子生物学鉴定。[结果]该茵细胞卵形，单极芽殖；在液体培养基中有沉淀且有茵环；在固体培养基中，茵落红色，表面平 滑、暗淡，边缘整齐，质地粘稠；无子囊孢子；无假茵丝形成。葡萄糖发酵为阴性；能同化硝酸钾；尿素分解呈阳性；明胶液化呈阴性；37℃ 呈阳性生长；DNA纯度较纯，核酸浓度为2 899,v,／ui。该酵母茵的18 S rDNA序列与粘性红圆酵母同源性达99．9％。[结论]形态特征及 生理生化和分子鉴定结果显示该茵为粘性红圆酵母。 关键词内生菌；形态学；生理生化特性；分子生物学；鉴定 中图分类号S182 文献标识码A 文章编号晒17—6611Ca00s)z5—10756—02 ’ Identification of DmDpl吨Strain n仪hlci鸣Red H删firom Fndt and Vegetable WANG Hong-qing (Xinyang A咖culhIml Higher College，Xinyang，Herman 46,1000) Abstract【Objective]The aim Was to identiS the endophyte stlain producing red pigment from fruit and vegetable．[Method]With the a_d叩1吨Slxllili from fruit and vegetable as tested material，its physiological and biochemical characteristics were甜nIy捌and its morphology and molecular biology were identified．[Result]The cell of the hlgi was oval and monopole budding．The f嘲had deposition and zone in liquid medium，its colony懈red with smooth and gray钏出ce，re出ed萨and sticky texture in solid inam，and the血r1矛had no ascospore and emdd not produce pseu[U讪．The缸姆 WaS negative tIⅢH曲sluco∞fermentand it could assimilate potassium nitrate．It showed positive tIIIul曲urBa hydrolysis and negative t}删gelatin liq— uefaetion．It also showed positive growth at 37℃．Its DNA purity was pure and iIS nucleic acid cotrlcn．was 2 899 ns／u1．卫1e homology of the 18 S rDNA sequence from the yeast w酗99．9％诵th that of the RIⅫ／otom／a僦比罾j脚．【Conclusion]The results of morphologic characteristics，physiological and biochemical and molectdar identification showed that the缸晒was R．，rnd如舒脚． Key words Endophyte；Morphologic；Physiological and biochemical characteristic；Molecular biology；Identification 1材料与方法 1．1材料 1．1．1菌种来源。形态学鉴定菌种来源：从果蔬中分离得到 的内生菌，低温保存于试管中备用；分子生物学鉴定菌种来 源：将待鉴定的菌种在麦芽汁平板上培养，通过平板划线分 离纯化单菌落。 1．1．2培养基。麦芽汁培养基、马铃薯培养基(m～)、葡萄糖发酵培养基、硝酸钾同化培养基、尿素培养基、糖发酵培养基、碳源培养基、明胶液化培养基。 1．1．3主要试剂。TE、10％SDS、5 mol／L NaCl、CRAB／NaCI、氯仿：异戊醇(24