Fromcytologytocellbiology.pptVIP

From cytology to cell biology Evolution driven by tools 一、Cytology-microscopic view of cell Tools: Light microscope Electron microscope Fluorescent microscope Confocal microscope 1.The Light Microscopy 2. Electron microscope Cytology-morphology  function of cells Cell morphology Histology immuno-histochemistry Cell division Mitosis and meiosis Cell organelles Primary function analysis Achievement of cytology Cell theory Chromosomes and gene Chromosome and cell cycles Gene theory Chromosome and inherited diseases Cell function Ultra-microstructure of cells 二、 Isolating Cells and Growing Them in Culture-Different faces Cell culture Primary cell culture ES cell culture Tissue culture Cell culture in suspension Hybrids cell culture-production of monoclonal antibodies 二、Isolating Cells and Growing Them in Culture-Features Uniform for analysis, good at consistence Easy to handle with less amount of agent ES cell maintained whole possibilities of differentiate to tissue of required (good perspective in many applications) Changing as they divide(be care of misleading to wrong conclusion!) Tumor cells are stronger than the normal cells Measuring cell content with fluorescent dyes PH Various ions Oxidant/antioxidant level Cell apoptosis Calcium (very important for excitable cells,binding of the dye lead to excite wavelength shift) 五、Molecular biology of cells Gene expression Activation of specific signal pathways Transcription factor Cell apoptosis Cell cycle Proliferation differentiation Molecular markers Gene expression at mRNA level PCR Real time PCR Regular PCR Northern blot In situ hybridization Other methods Gene expression at protein level Western blot Immuno-cytochemistry Elisa and similar Reporter gene 六、in vitro assay of gene’s function Delivery of specific gene to cells Inhibit gene expression RNAi Anti-sense RNA Overexpression of specific gene Assaying cell’s changes Single cell contained dye or stained with anti-body can be detected