第五章授粉和受精课稿.ppt

植物不经过雌雄配子融合而产生种子的生殖方式 The significance of apomixs? Advantages and disadvantages of propagation through seeds Hybrid production Summary 2. 自交不亲和性主要由S位点的基因控制，再辅以其它基因的共同作用 1. 助细胞引导花粉管向胚囊的伸长 3. GCS1是植物中首先发现的配子体识别因子 春风只在园西畔 荠菜花繁蜂蝶乱 David Twell en http://www2.le.ac.uk/departments/biology/people/twell/lab 读书报告（一） Assay to detect pollen tube attractant extracted from styles of Nicotiana tabacum, Cheung et al., 1995. Who guides the pollen tube going through the long style? 花粉管怎样经历漫长过程把精细胞送达目的地呢？ Style引导组织的AGPs：富含阿拉伯半乳糖的糖蛋白。 A G P s Style引导组织的AGPs：富含阿拉伯半乳糖的糖蛋白。 AGPs 引导花粉管的证明： 1）在活体的花柱中,AGP的浓度是从上至下渐升的 2）转录花柱特异性AGPs 的基因已克隆：TTS 。 35S:TTS反义cDNA A G P s 糖蛋白减少； 花粉管生长减缦； 受精率降低。 烟草 How pollen tubes fined the micropyles（珠孔）? 1) Ca2+ 2) Some information we do not know. How pollen tubes fined the micropyles? Directed growth of the pollen tube in Arabidopsis after emerging from the placenta 1) Ca2+ 2) Some information we do not know. 47H4 在拟南芥中与47H4类似的突变体还有54D12等。 54D12 的胚囊有各种不同的发育程度。 胚珠的受精频率与胚囊发育程度正相关,不能发育出胚囊的胚珠则完全不能吸引花粉管。 Directed growth of the pollen tube in Arabidopsis after emerging from the placenta 成熟胚囊里的那些细胞参与对花粉管的吸引？ Higashiyama T et al. (2001) used laser ablation teche in Torenia to solve the problem. They ablated … Pollen tube is attracted by two synegid cells（助细胞）. Chapter 16 Pollination and Fertilization 1. Self incompatibility 2. Pollen tube guidance 3. Fusion between gametes 配子体融合 Why the two sperms can find their lovers? A protein, GCS1, presents on the surface of generative and sperm cells of lily. 百合中发现GCS1在营养细胞和精细胞中表达 A single strong cDNA clone was detected in the generative cells of Lilium EM LM EB LB Gamete Cell GCS1 EF Bicellular Microspore Histidine-rich region 组氨酸区 Transit signal 信号传导区 Transmembrane 跨膜区 EM LM EB LB Gamete Cell GCS1 EF Bicellular Microspore A single strong cDNA clone was detected in the generative cells of Lilium Protein structure of GCS1 GCS1 expression in generative cells of Lilium a