生物化学 chap2 Protein 1幻灯片.pptVIP

Assignment for chapter 1 1.write out the three forms of α-D-fructose (open-chain, cyclization, Haworth projections). 2. Write out the molecule configuration of sucrose. 3. Write out the molecule configuration of amylopectin. (no less than 4 glucose residues) Chap 2 Protein Professional terms protein 蛋白质 amino acid 氨基酸 apellation 名称 isoelectric point （pI）等电点 peptide 肽 macromolecule/micromolecule 大分子/小分子 gene 基因 conversion coefficient of protein 蛋白质的换算系数 reciprocal 倒数 element 元素 crude protein 粗蛋白 amino group (-NH2) 氨基carboxyl group （-COOH）羧基 hydrophobic 疏水的 aromatic 芳香基团的 aliphatic 脂肪族的 polar 极性的 charged 带电荷的 uncharged 不带电荷的 Main contents The 20 standard amino acids (structure, apellation, symbols) Protein structure Important concepts and some professional terms (eg. isoelectric point, pI; peptide bond) Introduction Protein is a type of macromolecule composed of natural amino acids by peptide bonds. Variety (e.coli 3000; human bodies 100000; nature 1010-1012) Complex molecule structures and given biological functions important substance for the expression of genes Section 1 Chemical compositions Element C H O N S Conversion coefficient of protein(蛋白质的换算系数) nitrogen concentration of a protein sample maintains to be 15 to 17 percent, averaged 16 % reciprocal of 16%is 6.25 Meaning of 6.25 If the sample contains one gram of nitrogen element, then its protein concentration is 6.25 grams. For example If the N of a protein sample is determined to be 3.0 grams, calculate the crude protein concentration of the sample. Key: 18.75 g Crude protein / %=N%?6.25 Section 2 amino acids (AA) All proteins are made up of amino acids. Proteins of all species, are made up from the same set of 20 standard amino acids. (1) A primary amino group (-NH2) and a  carboxyl group (-COOH)(2) ?-carbon atom(3) if R=H, glycine(4) D-,L-amino acid 2.2 Classification of AA Hydrophobic, aliphatic R group Hy