从原料到产品生物基丁二酸生物合成研究进展-应用与环境生物学报.doc

从原料到产品：生物基丁二酸研究进展* 税宗霞1 秦 晗1 吴 波1 谭芙蓉1 王景丽1 何明雄1, 2** (1农业部沼气科学研究所，生物质能技术研究中心 成都 610041) (2农业部农村可再生能源开发与利用重点实验室 成都 610041) 摘 要 丁二酸是许多厌氧和兼性厌氧微生物的代谢中间产物。作为一种重要的有机化工原料及中间体，丁二酸广泛用于生物高分子、食品与医药等行业，市场潜在需求量巨大。同时，它作为一种优秀的C4平台化合物，被美国能源部认为是未来12种最具前景的生物炼制产品之一。相比传统的石化法生产丁二酸，微生物发酵法具有诸多显著优势。尤其是近年来随着石化资源的日益枯竭及环境污染问题的日益严峻，以生物质为原料生产丁二酸等生物基产品的研究备受国内外研究者的关注。因此，本文从产丁二酸菌种的种类及常见菌株产丁二酸的代谢途径、产丁二酸工程菌代谢工程技术构建策略及诱变筛选策略、丁二酸发酵过程控制与优化、丁二酸的分离提取工艺等4个方面综述了近年来国内外生物基丁二酸研究进展。最后对实现丁二酸高效低成本的生物制造进行了展望。图3表2参108 关键词 丁二酸；菌种选育；基因工程；代谢工程；过程强化；提取工艺 From Raw Materials to Products: Progress in Bio-based Succinic Acid SHUI Zongxia1, QIN Han1, Wu Bo1, TAN Furong1, WANG Jingli HE Mingxiong1,2 (1Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Section 4-13, Renming Rd. South, Chengdu 610041, China), (2Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Section 4-13, Renming Rd. South, Chengdu 610041, China) Abstract Succinic acid is one of the key intermediates in the tricarboxylic acid cycle (TCA) and has huge potentials in biopolymer, food, medicine applications. Succinic acid was identified as one of the top 12 building-block chemicals by the US DOE. On the other hand, production of succinic acid by microbial fermentation showed many significant advantages than traditional petro-chemical. Especially in recent years, with the increasing depletion of fossil resources and other environmental pollution problems, many researchers paid more and more attention on production of succinic acid and other bio-based products from biomass feedstocks. In this article, we reviewed recent research progress on the production of succinic acid by microbial fermentation, including the species of the succinic-acid-producing microbes, the progress of genetic engineering strategy and metabolic engineering technology for construction of succinic acid-producing strains, fermentation process control and optimization, and extraction process of s