基因重组蛛丝蛋白聚乙烯醇复合支架材料的制备-中华生物医学工程.doc

基因重组蛛丝蛋白-聚乙烯醇350007 福州 福建师范大学生命科学学院（魏梅红、涂桂云、李敏），化学与材料学院（陈登龙） 通信作者：李敏，Email:mli@fjnu.edu.cn 【摘要】 目的 探讨致孔剂NaCl粒径和比例、变性剂和聚乙烯醇（PVA）等因素对基因重组蛛丝蛋白- PVA复合支架材料形态及性能的影响。 方法 基因重组蛛丝蛋白溶解于98%甲酸，单纤维强力试验机多孔支架力学性能好断裂应力、断裂比强度均5倍，断裂伸长率可达12.21%以500 μm的NaCl为致孔剂多孔支架力学性能好PVA能明显改善重组蛛丝蛋白多孔支架的性能。 结论 重组蛛丝蛋白-PVA复合支架材料有望在组织工程领域得以应用。 【关键词】 重组蛛丝蛋白Preparation of recombinant spider silk protein/polyvinyl alcohol composite scaffold material WEI Mei-hong, TU Gui-yun, CHEN Deng-long, LI Min*. *College of Life Sciences, Fujian Normal University, Fuzhou 350007, China Corresponding author: LI Min, Email:mli@fjnu.edu.cn Snclusionslds ,China 【Abstract】 OTo investigate the effects of many factors such as porogen,denaturant and polyvinyl alcohol(PVA) polymer on the structure and mechanical of recombinant spider silk protein/PVA composite scaffold material. Methods The silk fibrous protein solution was prepared by dissolving the recombinant spider silk protein in 98% formic acid, recombinant spider silk protein / polyvinyl alcohol(PVA) composite porous scaffolds were prepared by freeze-drying／particle-leaching method. Scanning electron microscope was used to observe the conformation of the scaffolds, single strand strength testing machine was used to test the mechanical property of the scaffolds. Results It indicated that using ethanol as denaturant, the mechanical property of the scaffolds is better, the breaking stress and break specific tenacity enhances 5 times, the breaking elongation ratio can reach 12.21%. Using NaCl (φ500μm) as porogen, or the introduction of PVA can improve the performances of porous scaffold evidently. Conclusion Recombin