陈士林——中药dna条形码定方法体系的建立与推广陈士林——中药dna条形码鉴定方法体系的建立与推广陈士林——中药dna条形码鉴定方法体系的建立与推广陈士林——中药dna条形码鉴定方法体系的建立与推广.ppt

论文被Nature China选为中国最佳研究推介 A Plea for Pure Science (SCIENCE 1893/8/15) 假如我们只是留意科学的应用，我们很快就会退化成中国人那样—--多少代人以来他们都没有什么进步，因为他们只满足于科学的应用。 中国人知道火药应用已经若干世纪，如果他们探索其应用的原理，他们就会获得众多应用并发展出化学、物理学。 因为满足于火药能爆炸，而没寻根问底，中国人已经远远落后于世界的进步。我们现在只能将中国这个 最古老、人口最多的民族当成野蛮人。 美国物理学会第一任会长 Acknowledgements 课题组首次提出并验证ITS2条形码，引起国际广泛关注 国际期刊PLoS ONE关注药用植物DNA条形码。该文提出ITS2序列为药用植物通用的条形码序列，为陆地植物DNA条形码序列筛选提出了新的视角，由此引起同行的关注和讨论。 被德国专业网站选为近25年来在ITS2领域最受关注的16篇文章之一 http://its2.bioapps.biozentrum.uni-wuerzburg.de/ 该文发表后3年引文319次 Relative distribution of inter-specific divergence between congeneric species and intra-specific variation for four loci. The two bars in each box represent inter-specific (above) and intra-specific (below) genetic distances. Identification of the family Rosaceae W+ W- Relative Ranks, n, P value Result ITS2 matK W+ = 703, W- = 0, n = 37, P ≤1.139 × 10-7 ITS2 matK ITS2 rbcL W+ = 703, W- = 0, n = 37, P ≤ 1.137 × 10-7 ITS2 rbcL ITS2 rpoC1 W+ = 703, W- = 0, n = 37, P ≤ 1.133 × 10-7 ITS2 rpoC1 matK rbcL W+ = 620, W- = 46, n = 36, P ≤ 6.479 × 10-6 matK rbcL matK rpoC1 W+ = 655, W- = 11, n = 36, P ≤ 4.174 × 10-7 matK rpoC1 rbcL rpoC1 W+ = 359, W- = 106, n = 30, P ≤ 0.009 rbcL rpoC1 Wilcoxon signed-rank tests of inter-specific divergence among loci. ITS2 and rbcL, matK, rpoC1 were compared. For 54 samples from 43 species in 19 diverse genera, the successful identification rate with ITS2 was 100%, followed by matK (94%) and rbcL(65%). For 1410 samples from 893 species in 96 genera No. of genera (≥2 species): 47 (1351 samples) No. of species (≥ 2 samples ): 297 (814 samples) Identification rate with ITS2 was 100% and 78% at genus and species level, respectively. X Pang, Shilin Chen*. Cladistics, 2011, 27 Comparative analysis of a large dataset indicates that ITS should be incorporated into the core barcode for seed plantsChina Plant BOL Group, Li Dezhu et al., PNAS, 2011 We therefore propose that ITS/ITS2 should be incorporated i