积雪草苷对增生性瘢痕中转化生长因子-βmRNA及基质金属蛋白酶类.doc

积雪草苷对增生性瘢痕中转化生长因子-β mRNA及基质金属蛋白酶类表达的影响 作者：张涛1；荣新洲1；杨荣华1；利天增2；徐盈斌2　　(1广州市第一人民医院烧伤科，广东??广州??510180；2中山大学附属第一医院烧伤科，广东??广州??510080) 摘要：目的??探讨积雪草苷对人增生性瘢痕中转化生长因子β(TGF-β)mRNA和基质金属蛋白酶及其组织抑制剂(MMPS/ TIMPS)表达的影响。方法??临床上收集烧伤后5~8个月经积雪草苷治疗和非积雪草苷治疗的增生性瘢痕标本各9例。采用原位杂交和免疫组织化学方法，分别检测增生性瘢痕中TGF-β1 mRNA、TGF-β2 mRNA、TGF-β3 mRNA及MMP1、 MMP2、TIMP1、TIMP2和Ⅰ、Ⅲ型胶原的表达水平，并利用图象分析方法进行结果分析。结果 TGF-β mRNA在增生性瘢痕中主要表达于成纤维细胞胞质，经积雪草苷治疗的增生性瘢痕中TGF-β1 mRNA表达明显减少，胞质淡染，统计学上与非积雪草苷治疗组TGF-β1 mRNA表达有显著性差异(P0.01)；而TGF-β3 mRNA在积雪草苷治疗的增生性瘢痕中表达增多，明显高于非积雪草苷治疗组的TGF-β3 mRNA表达(P0.05)。MMPS/ TIMPS在增生性瘢痕中也表达于成纤维细胞胞质，经积雪草苷治疗的增生性瘢痕中TIMP1表达明显减少，与非积雪草苷治疗组TIMP1表达有显著性差异(P0.01)；而MMP1、MMP2、及TIMP2在上述两组中表达无显著性差异(P0.05)。Ⅰ、Ⅲ型胶原在增生性瘢痕中表达，经积雪草苷治疗的增生性瘢痕中Ⅰ型胶原排列整齐，含量较少，与非积雪草苷治疗组有显著差异(P0.05)；而Ⅲ型胶原在两组增生性瘢痕中表达无显著性差异(P0.05)。结论??积雪草苷通过降低TGF-β1 mRNA表达和增加TGF-β3 mRNA的表达，引起TIMP1表达明显减少，从而MMP1/ TIMP1相对比例增加，达到促进Ⅰ型胶原降解，减轻瘢痕增生的作用。 关键词：积雪草苷；转化生长因子β；基质金属蛋白酶；增生性瘢痕 中图分类号：R868??文献标识码：A??文章编号：1673-4254(2006)01-0067-04 Effect of asiaticoside on the expression of transforming growth factor-beta mRNA and matrix metalloproteinases in hypertrophic scarsZHANG Tao1；RONG Xin-zhou1；YANG Rong-hua1；LI Tian-zeng2；XU Ying-bin21Department of Burns, Guangzhou First Municipal People’s Hospital, Guangzhou 510180, China; 2Department of Burns, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaAbstract: Objective To investigate the effect of asiaticoside on the expressions of transforming growth factor (TGF)- β mRNA, matrix metalloproteinases (MMPS) and tissue inhibitors of metalloproteinases (TIMPs) in postburn hypertrophic scars. Methods Nine specimens of postburn (5-8 months) hypertrophic scars with asiaticoside treatment and 9 without asiaticoside treatment were collected for testing the expressions of MMPS, TIMPs, typeⅠand Ⅲ collagen and TGF-β mRNA by immunohistochemistry and in situ hybridization methods, followed by image analysis of the results. Results The expressions of TGF-β mRNA and MMPS /TIMPS were all detected in the fibroblast cytoplas