CuO―ENPs在三疣梭子蟹不同组织中的积累效应研究.docVIP

CuO―ENPs在三疣梭子蟹不同组织中的积累效应研究 　　摘要 为研究CuO-ENPs在三疣梭子蟹不同组织中的积累效应，选择三疣梭子蟹的鳃、血淋巴、肌肉、肝胰腺、心脏和胃作为研究对象，开展了CuO-ENPs在20、40 mg/L浓度下对三疣梭子蟹的慢性毒性试验，探讨了CuO-ENPs在不同组织内的积累效应。结果表明： CuO-ENPs的富集含量从高到低的顺序为 鳃血淋巴肌肉肝胰腺心脏和胃， 三疣梭子蟹暴露于不同CuO-ENPs浓度的条件下，体内CuO-ENPs的积累量具有一定差异，除血淋巴外，随着CuO-ENPs浓度的增高，不同组织的CuO-ENPs残留量有一定程度的增加，表现出明显的浓度效应。三疣梭子蟹鳃对CuO-ENPs的富集能力较强，在20、40 mg/L浓度下富集系数分别高达76.35和58.15，血淋巴对CuO-ENPs的富集系数分别为2.6和1.4，肌肉对CuO-ENPs的富集系数分别为1.77和1.43，肝胰腺对CuO-ENPs的富集系数分别为1.3和0.8，心脏和胃对CuO-ENPs的富集系数分别为1.30和0.65。三疣梭子蟹鳃对CuO-ENPs的富集作用远高于三疣梭子蟹的血淋巴、肌肉、肝胰腺和心脏及胃等组织。 　　关键词 CuO-ENPs；三疣梭子蟹；积累；富集系数 　　中图分类号 S968.25+2 文献标识码 A 文章编号 1007-5739（2016）19-0259-02 　　Abstract To study the accumulation effect of CuO-ENPs in different tissues of Portuns trituberculatus，gills，haemolymph，muscles，hepatop-ancreas，heart and the stomach of Portuns trituberculatus had been taken as objects in this study，and chronic toxicity test of Portuns trituberculatus had been carried out under the concentrations of CuO-ENPs of 20 mg/L and 40 mg/L，respectively.The results showed that the order from high to low of enrichment content of CuO-ENPs in different tissues of Portuns trituberculatus was gillhaemolymphmusclehepatopancreas heart and stomach.The enrichment content of CuO-ENPs in different tissues of Portuns trituberculatus was different with different concentration of CuO-ENPs.With the concentration of CuO-ENPs increased，the enrichment content of CuO-ENPs in different tissues was increased except for haemolymph.The enrichment ability of gills of Portuns trituberculatus was far higher than haemolymph，muscles，hepatopancreas，heart and the stomach.Under the concentrations of CuO-ENPs of 20 mg/L and 40 mg/L，respectively，the enrichment factors of gills were 76.35 and 58.15，respectively，while the enrichment factors of haemolymph were 2.6 and 1.4，respectively.The enrichment factors of muscles were 1.77 and 1.43，respectively.The enrichment factors of hepatopancreas were 1.3 and 0.8，respectively.The enrichment factors of heart and stomach were 1.30