The toxic effects of Tris-（2,3-dibromopropyl） isocyanurate（TBC） on genes expression of bmp2b and bmp4 of zebrafish embryos.docVIP

The toxic effects of Tris-（2,3-dibromopropyl） isocyanurate（TBC） on genes expression of bmp2b and bmp4 of zebrafish embryos 　　【Abstract】We exposed zebrafish embryos to Tris-（2,3-dibromopropyl）isocyanurate（TBC） at the concentration of 20ppb, 100ppb, 400ppb, 1000ppb for 120h and 0.1%DMSO was set as the control group. Bmp2b and bmp4 were chosen perform RT-PCR to determine their genes expression level. The results showed that, TBC influenced their genes expression level in some extent and it significantly raised the genes expression level at the concentration of 20ppb. 　　【Key words】Bmp; Gene expression; RT-PCR; Zebrafish 　　0　Introduction 　　Tris-（2,3-dibromopropyl） isocyanurate（TBC） is a new type of brominated flame retardants. It is stable and has high octanol-water partition coefficient. Since TBC was first detected from Liuyang River in Hunan province, China, in 1997[1], people started to study on its environmental toxicology. According to preliminary studies, TBC can damage zebrafish’s gill, liver, intestines, gonad and swimming bladder[1-2]. 　　Bmp signal pathway plays important role in the development of zebrafish. It involved in many aspects about vital actions, therein, Bmp2b and bmp4 are one kind of bmp subfamilies and are all expressed at early embryos[3-4]. In this study, we exposed zebrafish embryos to different concentration of TBC and through RT-PCR to determine the expression level of bmp2b and bmp4 to explore the influence of TBC on bmp signal pathway. 　　１　Materials and methods 　　1.1　Chemicals and animals 　　Tris-(2,3-dibromopropyl） isocyanurate（TBC, 97% purity） was purchased from Sigma, USA. The zebrafish（about 6 months） were bought from Nanshan market, Qingdao. Then, the fish were bred fresh Artemia nauplii twice a day in constant temperature room at 28℃ with the photoperiod about 14h light:10h dark. 　　1.2　Methods 　　1.2.1　Preparation of the solutions 　　The TBC stock solutions（20ppm, 100ppm, 400ppm,1000ppm） were prepared and then used zebrafi