不同黄瓜材料对烟粉虱的抗性及相关酶活性的研究.docVIP

不同黄瓜材料对烟粉虱的抗性及相关酶活性的研究 　　摘要：为筛选高抗烟粉虱的黄瓜新品种，本试验以“大青把”黄瓜为不抗烟粉虱对照（CK1），“无毛黄瓜”为抗烟粉虱对照（CK2），对国内外370份黄瓜种质资源进行烟粉虱抗性筛选，并对筛选出的抗性黄瓜材料接种烟粉虱，研究接种前后叶片中过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）的活性变化，以期为黄瓜烟粉虱抗性的鉴定提供理论依据。试验共筛选出四份高抗烟粉虱黄瓜材料，分别为4号、13号、19号、76号。其叶片经烟粉虱为害后，4号的PPO活性最高，13号的POD和PAL活性最高；4份材料的POD、PAL、PPO活性均极显著高于CK1。由此得出结论：不同黄瓜材料对烟粉虱的抗性与POD、PAL、PPO中的一种或几种物质的综合作用有密切关系。 　　关键词：黄瓜；烟粉虱；抗性；过氧化物酶（POD）；苯丙氨酸解氨酶（PAL）；多酚氧化酶（PPO） 　　中图分类号：S642.2文献标识号：A文章编号：1001-4942（2016）09-0065-04 　　AbstractTo screen new cucumber varieties with high resistance to Bemisia tabaci， using Daqingba cucumber as the non Bemisia tabaci-resistance control （CK1） and glabrous cucumber as the Bemisia tabaci-resistance control （CK2）， the resistances of 370 cucumber germplasm resources at home and abroad were screened. The chosen cucumber materials were inoculated with Bemisia tabaci， and then the activity changes of peroxidase （POD）， phenylalnine ammonialyase （PAL） and polyphenol oxidase （PPO） in the leaves before and after inoculation were studied to provide theoretical bases for the identification of Bemisia tabaci resistance of cucumber. The results showed that 4 cucumber materials with high resistance to Bemisia tabaci were screened out， including No.4， No.13， No.19 and No.76. After inoculation with Bemisia tabaci， the activity of PPO in the leaves of No. 4 cucumber was the highest； the activities of POD and PAL in the leaves of No. 13 cucumber were the highest； the activities of POD， PAL and PPO in the leaves of 4 anti-Bemisia tabaci materials were all very significantly higher than those of CK1. In conclusion， the resistance to Bemisia tabaci of different cucumber materials had close relationships with the synthetical effects of one or several substances of POD， PAL and PPO activities. 　　KeywordsCucumber； Bemisia tabaci； Resistance； POD；PAL；PPO 　　黄瓜（Cucumis sativus L.）属葫芦科黄瓜属，广泛分布于世界各地，是一种重要的蔬菜作物。粉虱是危害黄瓜的主要害虫之一，近年来，危害黄瓜的粉虱主要是烟粉虱（Bemisia tabaci）。烟粉虱俗称小白蛾，是一种世界性的害虫[1]，对其抗性研究是生物领域的热点之一。过氧化物酶（POD）能使组织中的某些碳水化合物转化成