中草药饲料添加剂诱导哈萨克羊发情规律研究.docVIP

中草药饲料添加剂诱导哈萨克羊发情规律研究 　　摘要 为了了解中草药饲料添加剂诱导哈萨克羊发情的生殖激素变化规律，采用酶联免疫法（ELISA）测定外周血液中E2、P4、MLT、FSH和LH等5种重要生殖激素的浓度。结果表明：血液中E2和P4呈现波动式变化，FSH和LH呈现规律性脉冲式变化。结果提示E2和FSH与绵羊的非繁殖季节发情有关，高浓度的P4和LH对开启或维持绵羊的发情有重要作用，为通过中草药饲料添加剂调控绵羊发情提供激素方面的理论依据。 　　关键词 中草药饲料添加剂；哈萨克羊；诱导发情；生殖激素 　　中图分类号 S816.7 文献标识码 A 文章编号 1007-5739（2016）10-0243-03 　　Study on Estrus Laws for Inducing by Chinese Herbal Medicine Feed Additive in Kazak Sheep 　　DANG Le 1 FU Xue-feng 2 SHI Gang 2 * 　　（1 The Animal Husbandry Station of Xinjiang Uygur Autonomous Region，Urumqi Xinjiang 830009；2 Institute of Animal Science，Xinjiang Academy of Animal Sciences） 　　Abstract The purpose of this study was to analyze reproductive hormones change rule of estrous kazak sheep，which were induced by Chinese herbal medicine feed additive.The concentration of reproductive hormones were measured by using enzyme-linked immunoassay（ELISA），including estradiol（E2），progesterone（P4），melatonin（MLT），follicle stimulating hormone（FSH） and luteinizing hormone（LH） of peripheral blood.The results showed that the E2 and P4 fluctuated type change，and the FSH and LH pulsed type change in blood.These results indicated that the concentration of E2 and FSH changed related to the anestrous season sheep estrous，and the high concentrations of LH and P4 played an important role for opening or maintaining sheep estrous.The study will provide the theory basis of reproductive hormones change rule by Chinese herbal medicine feed additive in regulate and control sheep estrous. 　　Key words Chinese herbal medicine feed additive；Kazak sheep；estrus induction；reproductive hormone 　　中草药饲料添加剂具有天然性、多功能性、无毒副作用、无抗药性等特性，是目前研发的热点[1-4]。中草药饲料添加剂可刺激母羊的生殖器官和调整母羊的生殖内分泌，诱导周岁羊当年发情，缩短周岁羊的发情周期、配种时间，达到快速提前交配以及促进排卵，提高产仔率以及产出率的目的[5]。 　　哈萨克羊为我国三大粗毛绵羊品种之一，具有较高的肉脂生产性能，以季节繁殖为主，产羔率103%[6]。本研究以季节性发情的周岁哈萨克羊为研究对象，检测补饲中草药诱导发情周岁羊与不发情周岁羊外周血液中雌二醇（estradiol，E2）、孕酮（progesterone，P4）、褪黑激素（melatonin，MLT）、促卵泡激素（follicle stimulating hormone，FSH）和促黄体素（luteinizing hormone，LH）等5种重要生殖激素的浓度，分析各生殖激素的浓度变化差异，以期阐