中国人血液淋巴细胞免疫表型参考值调查-流式细胞术论坛.docVIP

中国人血液淋巴细胞免疫表型参考值调查 作者：朱立华　…????文章来源：中华医学检验杂志????点击数： 702????更新时间：2005-9-26 【摘要】　目的　建立健康中国成人血液淋巴细胞免疫表型分析参考值。方法　用Simultest IMK-Lymphocyte试剂盒的荧光抗体对102例健康中国成人血液进行染色，以FACSort流式细胞仪进行分析，Simulset程序获取数据并分析结果，SPSS软件进行统计。结果　18～65岁健康中国成人淋巴细胞参考值范围CD+3细胞是50%～84%（955～2 860/μl），CD-3CD+19细胞为5%～18%(90～560/μl)，CD+3CD+4细胞为27%～51%(550～1 440/μl)，CD+3CD+8细胞为15%～44%(320～1 250/μl)，CD-3、CD+16和/或CD+56细胞为7%～40%(150～1 100/μl)，CD+3CD+4/CD+3CD+8比值为0.71～2.87。随着年龄增长CD+3CD+4细胞略有升高，CD+3CD+8细胞略有降低。NK细胞在大年龄组女性低于男性，且有统计学差异(P＜0.05)。与高加索人和美国人相比，中国人的CD+3CD+8细胞和NK细胞(CD-3，CD+16和/或CD+56)的相对值(百分率)与绝对值均高，CD-3CD+19细胞和CD+3CD+4细胞仅相对值较低，但绝对值不少。CD+3CD+4/CD+3CD+8比值向低值漂移。结论　种族、性别、年龄和环境因素可影响健康人淋巴细胞表型分布。 　　Reference values on blood lymphocyte immunophenotype in healthy Chinese adult 　Zhu Lihua, Wang Jianzhong, First Hospital, Beijing Medical University, Beijing, 100034 　　【Abstruct】　Objective　To establish the reference values on blood lymphocyte immunophenotype in healthy Chinese adults.Method　Staining whole blood with fluorescein-conjugated monoclonal antibodies supplied by simultest TM IMK lymphocyte kit, we analyzed 102 healthy Chinese residents (age range 18～65 years) by FACSort flow cytometer, acquired results by Simultest programme, and performed statistical analysis by SPSS software.Results　The 95% reference ranges in absolute counts per microliter of whole blood (percentage of lymphocytes) for CD+3, CD+3CD+4, CD+3CD+8, CD-3CD+19, CD-3/CD+16 and/or CD+56 cells were 955 to 2 860 (50% to 84%), 550 to 1 440 (27% to 51%), 320 to 1250 (15% to 44%), 90 to 560 (5% to 18%), 150 to 1 100 (7% to 40%) respectively. The CD+3CD+4/CD+3CD+8 ratio was 0.71 to 2.87. CD+3CD+4 cells increased and CD+3CD+8 cells decreased slightly with age. Women were compared to men in old age group, NK cells (CD-3/CD+16 and/or CD+56) were lower (P＜0.05). Compared with Caucasians and Americans, Chinese had higher percentage and absolute numbers of CD+3CD+8 and NK cells, but had lower percentage of CD-3CD+19 and CD+3CD+4 cells without absolu