专业英语---1讲述.ppt

Materials and methods the high-fat group Animal treatments the normal control group a high-fat control group a MG treatment group Sixty six 7-week-old male SD rats Materials and methods Animal treatments Blood samples Serum Portions of livers urinary samples Fecal samples Materials and methods Sample preparation. Urine samples were dissolved at room temperature prior to analysis, diluted 1:1 (v/v) with deionized water, vortexed for 60 s, and then centrifuged at 12000 rpm for 15 minutes. The clear supernatant was then transferred to an autosampler vial and kept at 4℃ 将尿样溶于室温下进行分析，用去离子水1:1（v / v）稀释，涡旋60s，然后在12000rpm，离心15分钟。 然后取澄清的上清液转移至自动进样器小瓶并保持在4℃。 Metabolic profiling Results Hyperlipidemia, characterized by increased TG, TC, LDL-C levels and decreased HDL-C levels. The TG, TC, HDL-C, LDL-C levels were measured. 高脂血症，其特征在于TG，TC，LDL-C水平升高和HDL-C水平降低。 所以要测量TG，TC，HDL-C，LDL-C水平。 Effect of MG on serum metabolites Results MG对血清中脂质水平的影响 Results Effect of MG on liver lipid and histopathology MG对肝脏中脂质水平的影响 Results MG在5,11,18周对高脂血症大鼠肝脏脂质聚集的影响 Results Effect of MG on fecal TG, TC MG对粪便中脂质含量的影响 Results Metabolomic analysis Results Discussion The first pathway is taurine and hypotaurine metabolism. The second pathway is glyoxylate and dicarboxylate metabolism. The third pathway is glycine, serine and threonine metabolism. The fourth pathway is the citrate cycle (TCA cycle). The fifth pathway is glycerophospholipid metabolism. The sixth pathway is primary bile acid biosynthesis. Discussion Conclusions In this study, a metabolomic approach based on UPLC-ESI-QTOF-MS detection has been successfully established for biomarker exploration in hyperlipidemia and mechanism studies of MG. Twenty metabolites were screened out and considered as potential biomarkers of hyperlipidemia. Taking these biomarkers as possible drug targets, it is revealed that MG could reverse the pathological process of hyperlipidemia through regulating the disturbed metabolism pathways. 在本研究中，