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中西医结合肝病杂志2007年第17卷第5期
2007 17 5
277
?ER???K?????????????????3 ????????????????
1231
11 21 31
( , 200032)
? ? ??:
( ER K)
?? ??:
, EL ISA Western
( ER K1/ 2)
1 (A P21)
ER K ( P2ER K)
P2ER K ,
, TN F2
,
??? ; ;
1/ 2 ;
21
KC ,
?? ??:
A P21 A P21
L PS (
L PS
?? :
( Kupffer cell , KC)
);
KC
100 g/ L ,
2
P2ER K ,
TN F2
ER K
1/ 2
; / ;/ ;
;
Effect of Qinggan Huoxue recipe and its separated recipe on ERK signaling
pathway in Kupffer cell mediated by L PS
W A N G M i ao , L O N G A i2hua , L I U Chen g2hai , et al1 L on g hua Hos pit al , S han g hai U ni versit y of T ra ditional Chi nese Medici me ( S han ghai , 200032) Chi na
Abstract Objective : To investigate t he effect of Qinggan Huo xue recipe and it s separated recipe on ER K signaling pat hway in Kupffer cells mediated by L PS. Methods : Kupffer cells were isolated f ro m t he livers of male Wistar rat s by in sit u perf usion wit h collagenase and Nyco ndenz density2gradient cent rif ugatio n , and were stimulated wit h L PS in differ2 ent do ses for different time to o bserve t he exp ressio n of TN F2 ; The drug serum of Qinggan Huo xue recipe and it s separated recipe were p repared and were added to t he medium. The levels of exp ression of TN F2 were evaluated by EL ISA . Western blot analysis were performed to detect p rotein exp ression of ER K1/ 2 and A P21. Results : After L PS was added to t he medium , t he p rotein exp ressio n of TN F2 increased. A significant effect was observed 2 hours after L PS stimulatio n , and reached it s peak 6 hours later wit h t he do se of 1mg/ L showing t he mo st evident effect . Qinggan recipe co uld down2regulate t he exp ressio n of membrane inhibit t he p ho sp horylatio n of ER K to supp ress t he exp ression of nuclear factor A P21. Huoxue recipe could decrease t he levels of p ho sp horylated ER K , lower t he exp ressio n of TN F2 . In a similar manner , Qinggan Huo xue recipe co uld regulat result in reduced p ho sp horylation of ER K and weaken exp res2 sio n of A P21. Conclusion :
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