分子检测技术在食品微生物及基因转殖食品之应用.PDFVIP

年年 類 Molecular Diagnostic Technique 1. Immunoassay technique RPLA (Reverse phase latex agglutination) ELISA (Enzyme linked immunoassay) RIA (Radio immunoassay) Immuno-PCR (Immunomagnetic bead PCR) Protein biochip 2. Gene detection method DNA probe: radioactive and non-radioactive probe PCR (Polymerase chain reaction) Multiplex PCR DNA microarray DNA chip 3. Molecular typing technique PFGE (Pulsed field gel electrophoresis) Ribotyping RAPD (Random amplified polymorphic DNA) RFLP (Restriction fragment length polymorphism) PCR-ribotyping General Assay Protocol 1. Sample Lysis Add Lysis Solution(s) to sample tubes and incubate to release rRNA target. 2. Hybridization Add Probe Solution and incubate. The DNA Probes hybridize to the rRNA target. 3. Hybrid capture Place dipsticks into tubes and incubate. Target-probe hybrids attach to the dipstick. 1/6 分子檢測 4. Enzyme label Wash dipsticks and place in tubes containing Enzyme Conjugate and incubate. 5. Hybridization Wash dipsticks and place in tubes containing SubstrateChromogen. Incubate for color development. Discard dipsticks. Add Stop Solution to tubes. 6. Detection Read absorbance at 450 nm on the GENE-TRAK? Photometer. Record the absorbance reading from the display. Product Name GENE-TRAK? Listeria Assay GENE-TRAK? Listeria DLP Assay GENE-TRAK? E. coli Assay GENE-TRAK? Salmonella Assay GENE-TRAK? Salmonella DLP Assay GENE-TRAK? Staphylococcus aureus Assay GENE-TRAK? Campylobacter Assay Format Description 100 pcs Colorimetric DNA hybridization 100 pcs Colorimetric DNA hybridization 100 pcs Colorimetric DNA hybridization 100 pcs Colorimetric DNA hybridization 100 pcs Colorimetric DNA hybridization 100 pcs Colorimetric DNA hybridization 100 pcs Colorimetric DNA hybridization ? Salmonella spp Salmonella Typhimurium, S. Enteritidis, S. Typhi, S. Choleraesuis, S. Schwarzengrund, etc ? 1 2 3 4 5 6 DAEC (Escherichia coli) (Enterotoxigenic E. coli, ETEC) (Enterohemorrhagic E. coli, EHEC) (Enteropathogenic E. coli, EPEC) (Entero