乌龙茶多糖的提取分离及降血糖活性研究.pdf

Science Nov．2006 5thChinese ofPharmaceutical TechnologyEducationSymposium Engineering 乌龙茶多糖的提取分离及降血糖活性研究 林强’张元’魏静娜2 1．北京联合大学生物化学工程学院，北京100023l2．天津大学化工学院，天津300072 关键词茶多糖酶处理膜分离四氧嘧啶降血糖 摘 要目的：用纤维素酶辅助提取、膜过滤法纯化乌龙荼多糖，以研究其降糖活性。方法：比较酶处理与否两种条件 下的提取量及微滤前后多糖的纯度，选择合适的提取分离路线。注射四氧嘧啶制造高血糖模型。灌胃给药28d，观察比较 茶多糖与其它几种成药的降糖效果。结果：增加酶处理过程后提取率提高31．91％，膜过滤使得粗茶多糖纯度由纯化前 小鼠有一定的治疗效果。 on of from andtheir Studyseparationpolysaccharidesoolong aClVltv laypoglycennc …-,IF L玎q Yuan‘，WEI Qian91。ZHANGJing·na2 1．Biochemical Union 100023，China； EngineeringCollegeofBeijingUniversity，Beijing 2．SchoolofChemical and 300072．China University,Tianjin EngineeringTechnology,Tianjin Abstract extractionandmembrane inthis andthenthe ObjectiveEnzyme-assisted sepatationtechnique(MST)wcreappliedpaper9 Effects Was afterthe effectsoftea ofMST hypoglucemicpolysaeeharide(TSP)obtained、Ⅳe化investigated；Methodsinvestigated oftraditionaland methodtoselectasuitable thenTSPandsomeotherkindsofmedicine compagison enzyme-assisted program,and were tomodelofdiabeticmellitusestablishedalloxanfor28dinorderto oftheir activities． lavaged byip comparehypoglycemic rate crude was ResultsCellulasetreatmentmakoa increaseofextraction31．91％andofthe significant by putity polysa