云南泡桐丛枝病植原体核糖体蛋白基因片段序列分析.pdf

植物病理学报 ACTA PHYTOPATHOLOGICASINICA 35(6)(ZK)：18-21(2005) 云南泡桐丛枝病植原体核糖体 蛋白基因片段序列分析 和志娇1，蔡红1，陈海如h，和根强2，吴华英1，唐丽明1 (1云南农业大学云南省植物病理重点实验窒，昆明650201；2云南省潞西市市农业局经济作物技术推广站，芒市678400) 行PCR扩增，得到1．3kb的特异片段，证明此病株中存在植原体。将此片段与pGEM—TEasy载体连接并转化大肠杆菌 白基因片段长l244 16S rI·B亚组的翠菊黄化(Aster yellows，AY)、长春花黄化(Periwinkle 原体株系属于翠菊黄化组B亚组(16Sfl—B)。 关键词：泡桐；丛枝；核糖体蛋白基因序列；植原体；翠菊黄化 ofribosomal of associatedwithPaulownia Sequencing proteingenephytoplasma witches’-broominYunnan Hai．m1，HE province脏Zhi-jia01，CAIHon91，CHEN Gen．qian92，WU forPlant ofYunnan (1 Laboratory Province，Yunnan Hua—yin91，TANGLi—min91Key Pathology AgriculturalUniversity， for Economic Bureauof 678400，China) Kunming650201，China；2StationPopularizingCrops，AgriculturalLuxi，Mangshi Abstract：Universal ribosomal wasusedtodetect of primer(rpFl_／rpPd)forproteingene phytoplasmapaulownia andDNA of1．3kbwas chainreaction fragment amplified．The witches-broom(PaWB—QJ)bypolymerase resultindicatedexistenceof associatedwiththediseased was phytoplasma plant．Theamplifiedfragmentliga— the cellofEcoliJMl09strain．ClonedDNA wereverifiedPCRandnucleo— tedinto competent fragments by strainconsists tide resultindicatedthattheribosomal of of sequenceanalysis．The proteingenePaWB-QJ 1244base containsentire revealedthat pairs，which rpsl9，rpl22and伊s3genes．Phylogeneticanalysis