新型电渗析电超滤集成过程在蛋白质浓缩与脱盐中的应用.pdf

新型电渗析-电超滤集成过程在蛋白质浓缩与脱盐中的应用 1，2 1 1 1 1* 宋伟杰 苏仪 陈向荣 沈飞 万印华 1 2 （中国科学院过程工程研究所生物化工国家重点实验室，北京 100190 中国科学院研究生院，北京 100049） 摘要：本实验采用自行设计的电渗析与电超滤集成（ED-EUF ）膜组件对牛血清白蛋白溶液的浓缩和脱盐过程进行了研究， 考察了电流强度，溶液浓度等因素对电渗析与电超滤集成过程的影响。实验结果表明，外加电场可以有效的抑制浓差极化 和膜污染，提高蛋白质的浓缩效率；同时由于离子交换膜和电渗析过程的引入，蛋白质溶液的盐含量也同时得到了降低。 提出的电渗析与电超滤集成过程，与传统的电超滤过程相比，在同样的电场条件下，可以同时实现蛋白质溶液的浓缩和脱 盐，进而提高外加电场的工作效率，是一个高效的集成过程。 关键词：电渗析，电超滤，蛋白质，浓缩，脱盐 Simultaneous concentration and desalination of protein solution by electrodialysis coupled with electro-ultrafiltration process SONG Weijie, SU Yi, CHEN Xiangrong, SHEN Fei, WAN Yinhua* The National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China Abstract: Concentration of BSA solution was performed using a home-made electrodialysis-electroultrafiltration membrane module. The effects of electric current, feed solution concentration on electrodialysis -electroultrafiltration (ED-EUF) process were investigated. Experimental results showed that the electric field could inhibit concentration polarization and membrane fouling, thus enhancing the efficiency of BSA concentration process. At the same time, the salt content in BSA solution could be decreased by the ion exchange membrane and electrodialysis process. As compared with conventional electro-ultrafiltration process, the novel ED-EUF process proposed in this article could achieve simultaneous concentration and desalinisation of BSA solution, thus promoting the efficiency of electric field. Keyword: electrodialysis, electro-ultrafiltration, protein, concentration, desalinisation 电超滤过程（EUF ）是利用外加电场对溶质的 超滤过程中的电极隔离膜分别放置于超滤膜的两 电泳效应，抑制浓差极化和膜污染，进而提高分离