溴代海因杀灭微生物的试验研究.pdf

H044 溴代海因杀灭微生物的试验研究 秦 娟，崔 群，胡 婷，李铭慧，姚虎卿 （南京工业大学化学化工学院，南京，210009 ） 摘 要 研究了二溴海因和溴氯海因水溶性制剂对大肠杆菌、枯草杆菌黑色变种芽孢和金黄色葡萄球菌的 杀灭情况；探讨了添加少量助溶剂对杀菌效果的影响。结果表明：以含溴氯海因632ppm 和514.1ppm 的 溶液对枯草杆菌黑色变种芽孢作用90 分钟，二溴海因574ppm 和474.4ppm 作用60 分钟，杀灭率均高达 99.7% 以上；助溶剂的加入可增强溴代海因水溶液杀灭微生物的能力；海因杀菌效果随放置时间延长而下 降；温度对溴代海因的杀菌效果有一定影响。 关键词：二溴海因; 溴氯海因; 杀菌效果; 助溶剂 Experimental Research on Germicidal Efficacy of Dibromohytoin and Bromochlorohydantion QIN Juan, CUI Qun, HU Ting, LI Ming-hui, YAO Hu-qing (College of Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing 210009, China) Abstract Efficacy test of dibromohydantoin and bromochlorohydantoin in killing Escherichia coli, spores of Bacillus var. niger and Staphylococcus aureus were carried out. Influence of dibromohydantoin and bromochlorohydantoin with little cosolvent in killing microbion was also discussed. The result showed that the killing rates of spores of Bacillus var. niger can reach out of 99.7% after exposure to the solution containing bromochlorohydantoin 632ppm and 514.1ppm for 90min and dibromohydantoin 574ppm and 474.4ppm for 60min. The addition of cosolvent can improve their germicidal efficacy. Their germicidal efficacy decreased with prolonging store time. The temperature had certain influence on their germicidal efficacy. Key words dibromohydantoin, bromochlorohydantoin, germicidal efficacy, cosolvent 二溴海因 （1，3-二溴-5，5-二甲基乙内酰脲）和溴氯海因 （1-溴-3 氯-5，5-二甲基乙内酰 脲）消毒剂是利用其水解产生的次溴酸 （HBrO ）和次氯酸 （HClO ），作用于微生物的细胞壁， [1] 使其通透性增加，细胞中的蛋白质与核心物质漏出，DNA 双链发生断裂，从而使细胞死亡 ； 具有强烈杀灭真菌、细菌及病毒的功效[2～6] ，是近期发展起来的高效、广谱、快速、安全的新 型氧化型消毒剂，可用于鱼、虾、蛙、甲鱼等水产养殖业中各种疾病的预防和治疗，还可用 1