LightCycler480系统特点和应用方向.ppt

快速循环模式 更快地升降温，提高反应速度，缩短反应进行的时间 快速循环模式 更快地升降温，缩短了可能的非特异性结合，提高PCR 的特异性 氙灯 CCD相机 5个激发通道 6个检测通道 优化光学组件排列设计 均一的激发及检测过程 LightCycler? 480荧光定量PCR系统 全能 可更换热循环模块：96 384 可采用多种样品数及体积：96（10-100 μl）；384（5-20 μl） 用不同光学滤镜组合可检测多种荧光染料：6个检测通道 可选多种检测模式：适用目前所有的检测模式 方便强大的软件工具 模块化软件 高速 快速热循环： Heating: (4.8) °C/s - Cooling: (2.5)°C/s 高性能试剂 定制耗材 LightCycler? 480荧光定量PCR系统 准确 数据生成: 全新的均一温控循环系统 数据捕获: 优化的均一检测光学系统 数据分析: 智能、可靠的算法 与仪器完美配合的耗材 自动化 预装软硬件随时整合自动化设备 LIMS/条码模块 带条码的多孔板 每个厂家都知道边缘效应，以及它的危害，也都在采取各种方法来降低或消除边缘效应的影响。 Serial 1:10 dilutions with 9 replicates of a plasmid DNA sequence were amplified with the LightCycler? 480 Probes Master and detected with a Universal ProbeLibrary probe. The PCR result shows a log-linear relationship over a broad dynamic range (10 log-intervals) and high reproducibility (Cp level) for replicates of the different dilution steps. Plasmid DNA, 20μl reaction. One amplicon containing two SNPs next to each other (neighboring bases in same codon); one probe pair is able to distinguish all combinations that occur in this sample set (note that double mutants with codon TAG were not present). Targte: Apolipoprotein B Detection with Hybridization Probes at 640 nm The LightCycler? 480 thermal block cycler is available for either 96- or 384-well plates; there are two catalog numbers for the instrument, depending on the block you need. Once installed, the block cycler is easily accessible and can be interchanged easily within minutes by users - without special training. This change also includes the block cycler cover (heated lied with pinholes). There is no requirement for an experienced service technician to do this. Each block cycler version is also available separately and comes in a specially designed loading help box. The upper part of the box is first placed next to the opened instrument; it can then accept the first block. The new block is inserted and the empty low