两种分析系统对活化部分凝血活酶时间测定结果的影响.doc

分析系统对活化部分凝血活酶时间测定结果的影响 李雪莲 江苏省连云港市第四人民医院检验科，江苏连云港 222000 摘要：目的 探讨不同检测系统对活化部分凝血活酶时间（APTT）检测结果的影响。方法 用鞣花酸和白陶土两种活化剂作为试剂分别在LG-PABER-1型血凝分析仪和Sysmex CA-500全自动血凝分析仪两种血凝仪上进行检测APTT。结果 ①在LG血凝仪上对41例标本进行APTT测定结果显示，白陶土测得结果大于鞣花酸测得结果（P 0.05）。 ②以鞣花酸为活化剂对41例标本进行APTT测定结果显示，LG血凝仪上测得结果大于Sysmex血凝仪的分析结果（P 0.05）。③在LG和Sysmex血凝仪上运用各自配套试剂对43例标本进行APTT测定结果显示，LG血凝仪上测得结果大于Sysmex血凝仪的分析结果（P 0.05）。④在LG和Sysmex血凝仪上运用各自配套试剂（放置不同时间试剂的混合试剂）对42例标本进行APTT测定结果显示，各仪器混合2试剂与混合1试剂两组数据间存在统计学差异（P 0.05）。结论 不同检测系统对APTT检测结果存在影响；不同仪器，不同试剂及不同试剂在不同仪器上检测APTT结果存在差异。建议不同的实验室应根据自己的活化剂和仪器建立自己的参考范围。 关键词：分析体系；活化部分凝血活酶时间；活化剂；影响因素 Effects of Different Analysis Systems on Activated Partial Thromboplastin Time(APTT) Test LI Xue-lian (Department of Clinical Laboratory, the Fourth People’s Hospital of Lianyungang, Jiangsu Lianyungang,222000,China) Abstract: Objective To investigate the influence of different analysis systems on activated partial thromboplastin time （APTT） test．Methods The APTT level of plasma was determined by ellagic acid and kaolin in blood clotting analyzers between LG-PABER-1 analyzer and Sysmex CA-500 analyzer.Results ①The APTT levels of 41 cases specimen were tested by ellagic acid and kaolin in LG-PABER-1 blood clotting analyzer．Result of kaolin group was much higher than result of ellagic acid group (P0.05)．②The APTT levels of 41 cases specimen were tested by the same ellagic acid with the same calcium chloride in LG blood clotting analyzer and Sysmex analyzer．LG analyzer’s result was much higher than Sysmex analyzer’s (P0.05)．③The APTT levels of 43 cases specimen were tested by every analyzer’s reagent in LG blood clotting analyzer and Sysmex analyzer．LG analyzer’s result was much higher than Sysmex analyzer’s (P0.05)． ④The APTT levels of 42 cases specimen were tested by every analyzer’s reagent that was a mix liquid,was used different time in LG blood clotting analyzer and Sysmex analyzer．The result was that there was marked difference between mix 2 reagent and mix 1 reagent in LG analyzer and Sysmex an