树舌灵芝多糖的分离纯化及抗氧化活性研究讲述.doc

安徽大学江淮学院 本科毕业论文 学生姓名：　　张珊　　 学号 系 别： 理工部　　　专业：　　生物科学 入学时间：　 　　2012　　年　　　09　　月 导师姓名：　　吴庆喜　　职称/学位：　 讲师 导师所在单位：　　 安徽大学　　　　 完成时间：　 　　　2016　　　　年　　　　04　　　　月 树舌灵芝多糖的分离纯化及抗氧化研究 摘 要 β-葡萄吡喃糖，单糖组成得到五种单糖的摩尔比为1：3.3: 6.2：14.35：17。结论：用超滤得到的单一组分的多糖GT-2分子量较小，蛋白质含量较少，具有典型的多糖吸收峰，由甘露糖，葡萄糖醛酸，葡萄糖，半乳糖，阿拉伯糖组成，抗氧化研究表明具有很强的抗氧化能力。 关键词：树舌灵芝；超滤；抗氧化作用 Purification and Antioxidant Activity of Ganoderma lucidum Polysaccharides Isolated Tree Tongue Summary Objective: To study the water-soluble polysaccharide Purification and antioxidant effects. Methods:The traditional water extraction and alcohol precipitation method obtained crude polysaccharide, were isolated and purified by ultrafiltration method, single component polysaccharide, and carries on the simple characterization analysis from the size of the molecular weight, infrared spectroscopy, monosaccharide composition, the further study of the single component polysaccharide on hydroxyl radical, DPPH free radical scavenging ability.Result: Separation and purification of get single group of sugar GT-2 molecular weight is 5.04 x 103 and infrared spectrum analysis showed that Ganoderma applanatum polysaccharide GT-2 is typical of the polysaccharide absorption peak is beta - Grape pyranose and monosaccharide compositions were the molar ratio of five kinds of monosaccharide 1:3.3: 6.2:14.35:17. Conclusion: Obtained by ultrafiltration single group of polysaccharide GT-2 molecular weight smaller, less protein content, with the typical absorption peak of the polysaccharide, composed of mannose, glucuronic acid, glucose, galactose, arabinose, antioxidant research show has very strong antioxidant ability. Keywords:Ganodermalucidum;Ultrafiltration;Antioxidant;Characterization and Analysis 目录 1　引言 1.1　树舌灵芝的研究 灵芝又称灵芝草、神芝、芝草、仙草、瑞草，是多孔菌科植物赤芝或紫芝的全株。根据我国第一部药物专著《神农本草经》记载：灵芝有紫、赤、青、黄、白、黑六种，性味甘平。灵芝原产于亚洲东部，中国古代认为灵芝具有长生不老、起死回生的功效，视为仙草。 树舌灵芝为灵芝科（Ganodermatacea