毕业设计：牛初乳中乳铁蛋白提取工艺的研究讲述.doc

青 岛 科 技 大 学 毕 业 设 计 （论 文） 题 目 __________________________________ __________________________________ 指导教师__________________________ 辅导教师__________________________ 学生姓名__________________________ 学生学号__________________________ ______________________________学院 _______________应用化工技术__________专业________________班 ______年 ___月 ___日 牛初乳中乳铁蛋白提取工艺的研究 摘 要 牛初乳是指健康奶牛分娩后1周内所分泌的乳汁，它不仅含有 丰富的营养物质，还含有多种免疫因子和生长因子等活性成分，具有机体免疫力、调节肠道菌群、改善胃肠道功能、促进生长发育、增强智力、调节血糖、改善糖尿病症状等多种保健功能。牛初乳作为功能性食品的重要原料，具有广阔的开发利用前景。从牛初乳中分离提取乳铁蛋白 , 建立快速分离纯化乳铁蛋白的工艺. 选择弱酸性阳离子交换剂 CM2SEP HA ROSE FAST FL OW 装柱 ,脱脂牛初乳在 4 ℃下进行动态吸附上柱后进行阶跃洗脱. 通过对洗脱条件的比较优化 ,确定了分离提取乳铁蛋白的最佳工艺条件为:牛初乳料液 p H 6 . 6 ,上柱速度 300 mL/ h ,阶跃洗脱剂的体积分数分别为 1 . 5 %和 5. 0 %的 NaCl ,洗脱体积流速 200 mL/h. 乳铁蛋白洗脱液经过超滤和冷冻干燥 ,得到冻干粉纯 度可达 98 . 98 % ,得率为 29 . 0 %. 关键词：牛初乳；乳铁蛋白 ；营养价值；保健功能；开发利用；离子交换;纯化 Bovine colostrum lactoferrin in extraction process of research ABSTRACT Bovine colostrum is the secretion of milk by healthy cows within 1 week after delivery．It is not in nutrients，but also contain a wide range of active ingredients such as immune factors and growth factors．Bovine colostrum showed various effective function such as strengthening organic immunity from disease，adjusting intestinal flora，improving tract function，stimulating physical growth，enhancing intelligence，regulating blood sugar，improving diabetes symptoms and so on．It is important raw material of functional food． To purify high purity (L F) from bovine , a fast n technique by t n exchange process. Weak a CM SEP HA ROSE FA S T FL O W was selected . At 4 ℃,t he skim milk was adsorbed dynamically to t he , and t occurred in two stages . The op L F p H 6 . 6 , sample flow rate of 300 mL / h , 1 . 5 % and 5 . 0 % NaCl as t he ant respectively in t he two stages and the velocity of 200 mL / h . The purity of L F pro duct reached 98 . 98 % and t he total yield of L F was 29 . 0 %. KEY WORDS: bovine colostrum；nutritional value；health function；utilization ；bovine colostrum ; 目