HPLC同时测定甘薯茎叶中叶黄素和β–胡萝卜素.PDFVIP

湖南农业大学学报(自然科学版) 2015 年 10月 第 41卷 第 5期 Journal of Hunan Agricultural University (Natural Sciences), Oct. 2015, 41(5):533–537 DOI:10.13331/ki.jhau.2015.05.015 投稿网址： HPLC 同时测定甘薯茎叶中叶黄素和 β–胡萝卜素 邢鹏 1，包兢兢 1，秦玉芝 1，陆英 1,2* (1.湖南农业大学园艺园林学院，湖南 长沙 410128；2.国家植物功能成分利用工程技术研究中心，湖南 长沙 410128) ? 摘 要：以水果薯、水果花叶薯、紫羽 1号、紫羽 2号、农大紫 1号、农大紫 2号、浙紫 1号、紫薯 2号、高淀 粉、叶用薯 1 号、叶用薯 2 号等 11 种不同品种甘薯茎叶为试验材料，建立了高效液相色谱法同时测定甘薯茎叶 中叶黄素和 β–胡萝卜素含量的方法。结果表明：采用甲醇为提取溶剂，料液比 1∶30，50 ℃超声提取 3次，每次 40 min，能较完全提取甘薯茎叶中的叶黄素和 β–胡萝卜素；HPLC以甲醇–水(95∶5)、甲醇–乙腈–四氢呋喃(70∶ 20∶5)为流动相梯度洗脱，叶黄素和 β–胡萝卜素分离良好，回收率分别为 105.8%和 101.5%；叶黄素及 β–胡萝卜 素含量最高的是甘薯茎叶中的成熟叶，其次为新叶，茎的含量最低；成熟叶中叶黄素与 β–胡萝卜素的含量分别在 0.504~1.031 mg/g和 0.188~0.467 mg/g，新叶中叶黄素与 β–胡萝卜素的含量分别在 0.409~0.617 mg/g和 0.141~0.242 mg/g，叶柄中叶黄素与 β–胡萝卜素的含量分别在 0.094~0.195 mg/g和 0.040~0.082 mg/g，茎中叶黄素与 β–胡萝卜 素的含量分别在 0.053~0.103 mg/g和 0.036~0.045 mg/g。 关 键 词：甘薯茎叶；高效液相色谱；叶黄素；β–胡萝卜素 中图分类号：S531.01 文献标志码：A 文章编号：1007?1032(2015)05?0533?05 HPLC analysis method on Lutein andβ-carotene in sweet potato(Ipomoea batatas Lam) leaves Xing Peng1, Bao Jingjing1, Qing Yuzhi1, Lu Ying 1,2* (1.College of Horticulture and Landscape, Hunan Agricultural University , Changsha 410128, China; 2.National Research Center of Engineering Technology For Utilization of Functional Ingredients From Botanicals, Changsha 410128，China ) Abstract: Stems and leaves of 11 sweet potato varieties ( Shuiguo, Shuiguo-huaye, Ziyu 1, Ziyu 2, Nongda-zi 1, Nongda- zi 2, Zhezi 1, Zishu 2, Gaodian-fen, Yeyong 1 and Yeyong 2 ) were used as material, to study the method of HPLC for the determination of lutein and β-carotene simultaneously. The results showed that lutein and β-carotene could be extracted from sweet potato leaves by ultrasound extracted with 1∶30 of materials to methanol at 50 ℃ three times , each time with 40 min. With HPLC mobile phase of methanol-water(95∶5, v/v) and methanol-acetonitrile-tetrahydrofuran (70∶ 20∶5), lutein and β-carotene could be separated well and their recoverys were 105.8% a