蛋白质组学介绍解析.ppt

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * The Mass (DA) of the amino acids Symbol Structure Mass (Da) Ala A -NH.CH.(CH3).CO- 71.0 Arg R -NH.CH.[(CH2)3.NH.C(NH).NH2].CO- 156.1 Asn N -NH.CH.(CH2CONH2).CO- 114.0 Asp D -NH.CH.(CH2COOH).CO- 115.0 Cys C -NH.CH.(CH2SH).CO- 103.0 Gln Q -NH.CH.(CH2CH2CONH2).CO- 128.1 Glu E -NH.CH.(CH2CH2COOH).CO- 129.0 Gly G -NH.CH2.CO- 57.0 His H -NH.CH.(CH2C3H3N2).CO- 137.1 Ile I -NH.CH.[CH.(CH3)CH2.CH3].CO- 113.1 Leu -NH.CH.[CH2CH(CH3)2].CO- 113.1 Lys K -NH.CH.[(CH2)4NH2].CO- 128.1 Met M -NH.CH.[(CH2)2.SCH3].CO- 131.0 Phe F -NH.CH.(CH2Ph).CO- 147.1 Pro P -NH.(CH2)3.CH.CO- 97.1 Ser S -NH.CH.(CH2OH).CO- 87.0 Thr T -NH.CH.[CH(OH)CH3).CO- 101.0 Trp W -NH.CH.[CH2.C8H6N].CO- 186.1 Tyr Y -NH.CH.[(CH2).C6H4.OH].CO- 163.1 Val V -NH.CH.[CH(CH3)2].CO- 99.1 Protein identification What is the sequence of the 624 component? m/z 624 521 406 293 130 43 ▲ 103 115 115 163 87 aa Cys Asp Asp Tyr Ser The difference in the m/z valve gives you the identity of the corresponding amino acid So the peptide sequence for the 625 component is: Cys-Asp-Asp-Tyr-Ser THE END! * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Overview of proteomics Summary time! Spend 15- 20 mins going over this as a group MAKE NOTES!!!!!!!!!!!! /cfapps/research/data_admin/Site602/mainpageS602P0.html Mass Spectroscopy Mass Spectroscopy An analytical technique used to measure the mass-to-charge ratio of ions. It is most generally used to find the composition of a physical sample by generating a mass spectrum representing the masses of sample components. The technique has several applications, including: 1) identifying unknown compounds by the mass of the compound molecules or their fragments Mass Spectroscopy 2) determining the isotopic composition of elements in a compound. 3) determining the structure of a compound by observing its fragmentation 4) quant