白芍、赤芍化学成分差异及其对大鼠胸动脉平滑肌A7r5细胞的生物效应比较.pdfVIP

J South Med Un叫 2013， 33(10) : 1453-1457 doi 10.3969jj.issn.1673-4254.2013.10.09 1453 基础研究 自苟、示苟化学成分差异及真对大鼠胸动脉平渭肌 A7r5细胞的 生物娘应比较 朱雅玲，周若龙，吴金雄，张 娴，杨柳 广州中医药大学第二附属医院H广东省中医药科学院，广东广州 5 10120 摘要 : 目的比较臼苟、赤均水提物中所含化学成分部J异同 ，及探讨白苟、赤苟水提物作用于体外大f蚓句动脉平滑肌株(A7r5)的 生物效应差异。 方法通过HPLC及质i曾分析，比较白苟、赤苟水提物中各单体化学成分的共性与差异。 采用体外培养A7r5 ，利 用血小板源生长因子BB(POOF BB)诱导A7r5建立细胞增殖病理模型，加入不同剂量的臼苟、赤苟水提物 ，通过MTI 比色法和 实时细胞分析仪检测细胞活性。 结果 白苟、赤苟在化学成分上有差别 ， 即使两者拥有共同的成分，含量亦存在差异。 在A7r5 增殖研究显示 ，300 μglml 白苟水提液与 A7r5对照组相比，细胞增殖显著增加(PO.OI) ;而赤苟71提液对其增殖并不显著(P 0.05)0 100-500μg/ml浓度的赤苟)j(:提物与PDGF BB病理模型组相比 ，细胞增殖有显著抑制(PO.OI ) ;而臼苟水提液对其增殖 并不明显(P0.05) 。 结论 臼苟、赤苟))提物在化学成分上存在差异，并在细胞活性研究中，两者亦表现出不同的统物效应。 关键词 : 白苟;赤苟 ; A7r5;PDGF BB;MTI Comparison of ch emical components between Baishao and Ch臼hao water extrac恒 and their effects on p roliferation of rat 出oracic ao此a smooth muscle cells ZHU Yaling, Yeuklung Chow, WU Jinxiong, ZHANG Xian, YAN G Liu Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China Abstrad: Objective T，。∞mpa1e the chemical compono四ts il1 Baislωo and CllishllO wate, extracts al1d inves挝gate their effects on the p1olife1a创。n of 1at tho1a 口c aorta smooth musc1e cells in vi打0. Methods τ11e cont.四ts and chemical st1uctu1es of monomers sep盯ated from t1飞e water ext1acts of Baishao and Chishao were analyzed using high-pedo1mance liquid ci1romatog1aphy and mass spec柱。s∞P予 Rat tho1acic aOl1:a smooth musc1e cell lir回 A715 and its platelet- de1ived growth facto1- BB (pDGF- BB)- il1duced p1olifelation model w町e exposed to diffe寄自lt concel1t1ati回回。f Baishao 缸飞d Chishao wate1 extracts, and the cell viability was 缸1alyzed by mitochol1drial-dep目叫臼飞t reductiol1 of lvπT 缸飞d real-time cell analyze1. Results The grow位也飞。ofA7乃15 C叫ellsw码 s曰吨2喀gnific臼an甜.tly s州挝tim~口飞u旧飞u巾1 (仲O.历). 111 PDGF-BB-induced cell p1oli但1ation model, the cell gowth was significantly SUppl白白d by 100-500 !-lglml Ch臼hao wat臼. ext1act (PO.Ol), while Baishao water exuact showed no obvious effect 011 the cell p1oli阳甜∞ (