胶束增敏催化分光光度法测定痕量铜 大学论文.doc

毕业设计(论文) 课 题 名 称 胶束增敏催化分光光度法测定痕量铜 学 生 姓 名 / 学 号 / 院 系 、专 业 生物与化学工程系化学专业 指 导 教 师 /老师 职 称 副教授 2008年 5 月 28 日 摘 要 （ 邵阳学院生物与化学工程系） 本文研究了在0．1 mol／L3.H2O介质中，痕量Cu(Ⅱ)催化过氧化氢氧化溴甲酚绿褪色反应，建立了催化分光光度法测定铜的新方法该方法具有高灵敏度和高选择性，并对该体系的最佳实验条件进行了研究探讨。研究表明：在阳离子表面活性剂十六烷基三甲基溴化CTMAB）存在的条件下，二价铜离子催化过氧化氢氧化溴甲酚绿褪色反应，该体系的最大吸光度对应的吸收波长为624 nm。Cu(II)线性范围为0.50-1.10 μg／ml，检出限为2.65×10-3μg／mlCTMAB，痕量分析 Abstract Liu Jinmei (The Chemistry of Grade 2004 Department of Biology and Chemistry Engineering of Shaoyang University) A new catalytic spectrophotometric method for the determination of trace Cu(II) was established with high sensitivity and selectivity in this paper.The method was based on catalytic effect of Cu(II) on the oxidation of 3,3′,5,5-Tetrabromo-m-cresol sulfonaphthalein by hydrogen peroxide in the medium of 0．1 mol／LNH3.H2O. The optimum conditions of the coloring reaction were studied．It was found that Cu(II) reacts with 3,3′,5,5-Tetrabromo-m-cresol sulfonaphthalein on the condition of CTMAB to form a stationary complex with maximum absorption wavelength at 624nm．The linear range of edeterminatlon is 0.50—1.10μg／25ml.．The detection limit in this method is2.65×10-3μg／ml. The method has been applied to the determination of trace copper in the water samples with satisfactory results． Key words：copper ；glues ；Catalytic spectrophotometry; 3,3′,5,5-Tetrabromo-m-cresol sulfonaphthalein； cetyltrimethylammonium bromide； trace analysis.  目录 ------------------------------------------------------------------------------2 英文摘要--------------------------------------------------------------------------------2 1 引言-------------------------------------------------------------------------------4～5 1.1 目的意义--------------------------------------------------------------