显微镜检查与干化学试纸条检测尿管型的临床应用.pdfVIP

·临床研究· 显微镜检查与干化学试纸条检测尿管型的 临床应用 许建邦 张晓坤 关达光 刘干辉 【摘要】目的 探讨用 3 种显微镜检查法与 2 种干化学试纸条检测尿管型的临床应用价值。 方法 按NCCLS文件GP16－A和中华医学检验分会文件《尿沉渣检查标准化的建议》要求，收集 1236 份临床尿液标本，同时用DiaSys R/S 2003 尿沉渣定量分析工作站法、非染色尿沉渣镜检法、过筛 法和Multstix TM 10SG，Combur 10 TEST?M两种干化学试纸条进行分析。结果 3 种镜检方法检出管型 阳性率：工作站法 8.25%、离心法 4.77%、过筛法 2.99%，与 2种干化学试纸条蛋白区带的阳性符合 率：工作站法 35.3％～43.1％、离心法 42.4％～49.2％、过筛法 48.6％～51.4％；阴性符合率： 工作站法 90.7％～94.7％、离心法 89.8％～94.0％、过筛法 89.2％～93.5％；误诊率：工作站法 5.3％～9.3％、离心法 6.0％～10.2％、过筛法 6.5％～10.8％；漏诊率：工作站法 56.9％～64.7 ％、离心法 50.8％～57.6％、过筛法 48.6％～51.4％。结论 应用DiaSys R/S 2003 尿沉渣定量分 析工作站法能提高管型检出率；干化学试纸条对管型检测有提示作用，其出现误诊率和相当高漏诊 率应予高度重视；选用优质试纸条能相应提高管型检出符合率和降低漏诊率。 【关键词】尿沉渣显微镜检查；尿干化学法检查；蛋白；管型 The clinical application on the examinations of the urine casts with the examination of microscope and reagent stripe. Xujianbang, Zhangxiaokun, ,Guandaguang，Liuganhui；1、The second Affiliated Hospital,Guangzhou Medical College,Guangzhou 510260,China.2 、 Department of clinical laboratory,Liwan Hospital,Guangzhou Medical college,Guangzhou 510170,China. [Abstract]: Objective To investigate the application worth among three methods of microscope and two methods of reagent stripe. Methods collecting 1236 cases of clinical urine samples under the requirement of NCCLS GP16-A,Analysis urine casts with the Diasys R/S 2003 urine sediments analysis workstation (Diasys),non-stain miscroscopic examination (NSME),Filtration examination (FE) and two methods of reagent stripe of MultistixTM 10 SG and combur 10 Test? M. Result Three methods of Microscope exmination and two methods of reagent stripe show the rate of positive conformability: Diasys was 35.3%～43.1%、NSME was 42.4%～49.2%、FE was 48.6%～51.4%; the rate of negative conformability: Diasys was 90.7%～94.7%、NSME was 89.8%～94.0%、FE was 89.2%～93.5%; the rate of erroe diagnostic: Diasys was 5.3%～9.3%、NSME was 6.0%～10.2%、FE was 6.5%～10.8%; the rate of lost diagnostic: Diasys was 56.9%～64.7%、NSME was 50.8%～57.6%、FE was 48.6%～51.4%. Conc