Cloning of new members of heat shock protein HSP101 gene family in wheat.pdf

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Cloning of new members of heat shock protein HSP101 gene family in wheat.pdf

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Cloning of new members of heat shock protein HSP101 gene family in wheat

Cloning of new members of heat shock protein HSP101 gene family in wheat (Triticum aestivum (L.) Moench) inducible by heat, dehydration, and ABA1 Janee L. Campbell a;2;3, Natalya Y. Klueva a;3, Hong-gang Zheng a;3;4, Jorge Nieto-Sotelo b;5, T.-H.D. Ho b, Henry T. Nguyen a;* a Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA b Department of Biology, Washington University, St. Louis, MO, USA Received 24 April 2000; received in revised form 17 November 2000; accepted 21 November 2000 Abstract We have cloned two cDNAs, TaHSP101B and TaHSP101C, encoding two heat stress-inducible members of HSP101/ClpB family in bread wheat (Triticum aestivum (L.) Moench.). Proteins encoded by these cDNAs are highly similar at the primary sequence level and diverged from the previously reported TaHSP101 (designated TaHSP101A) both in the consensus ATP/GTP-binding region II and in the carboxy terminal region. The HSP101 gene was determined to be a single copy gene or a member of a small gene family in hexaploid wheat. Messages encoding HSP101 proteins were inducible by heat stress treatments in both wheat leaves and roots. Accumulation of the TaHSP101C mRNA was less abundant than that of TaHSP101B mRNA. We are showing for the first time that in addition to heat stress, expression of HSP101 mRNAs in wheat leaves was induced by a 2-h dehydration and a treatment with 5U1035M ABA, but not affected by chilling or wounding, indicating that HSP101 proteins may be involved in both heat and drought responses in wheat. ? 2001 Elsevier Science B.V. All rights reserved. Keywords: Heat shock protein; Heat stress; Drought stress; ABA; (Triticum aestivum) 1. Induction Induction of heat shock proteins (HSPs) is one of the most universal and rapid responses of all cells to heat stress. Recently, it was demonstrated that proteins belong- ing to HSP100 family are involved in the process of the acquisition of thermotolerance [1,2] and their intracellular function is to aid i