有哪些信誉好的足球投注网站Development and evaluation of colloidal gold immunochromatographic strip for detection of Escherichi.pdf
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Development and evaluation of colloidal gold immunochromatographic strip for detection of Escherichi
African Journal of Microbiology Research Vol. 4(9), pp. 663-670, 4 May, 2010
Available online /ajmr
ISSN 1996-0808 ? 2010 Academic Journals
Full Length Research Paper
Development and evaluation of colloidal gold
immunochromatographic strip for detection of
Escherichia coli O157
Xihong Zhao1, Xiaowei He1*, Wenmei Li 1,3, Yao Liu2, Liansheng Yang1 and Jihua Wang3
1College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
2Zhongshan Supervision Testing Institute of Quality and Metrology, Zhongshan 528403, China.
3Guangzhou Wondfo Biotech CO., LTD, 510530, Guangzhou, China.
Accepted 24 February, 2010
Escherichia coli O157:H7 is a serious and common human pathogen that can cause diarrhoea,
haemorrhagic colitis, and haemolytic uraemic syndrome (HUS). In this study, the synthesis and
identification of colloidal gold particles and antibody-colloidal gold conjugates probe specific to E. coli
O157:H7 were performed, and the preparation of colloidal gold immunochromatographic strip based the
biotin-streptavidin system was developed for detection of E. coli O157:H7.Monodispersional nanogold
colloid was synthesized and preparation of nanogold-labeled polyclonal antibody probe to E. coli
O157:H7 by citrate method. Combination of antibody with nanogold particles was also characterized by
UV-visible (UV-vis) light absorption spectra and transmission electron microscopy (TEM). Furthermore,
nanogold-labeled probe was used to develop an immunochromatographic (IC) strip for E. coli O157:H7
analysis. With this method, analysis could be completed in less than 10 min. Examination of the 65
known strains (36 E. coli O157 strains and 29 serotypes other than E. coli) showed 98.5% specifity and
100% sensitivity, only yield a false-positive reaction with Salmonella choleraesuis. The sensitivity of the
IC strip was tested using 10-fold dilution E. coli O157 in foods, could be detected at a minimum of 2.3
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