Development of an immunochromatographic lateral flow test strip for.pdfVIP

下载本文档

10
0
约2.68万字
约 7页
2017-04-06 发布于江苏
举报
版权申诉

Development of an immunochromatographic lateral flow test strip for.pdf

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Development of an immunochromatographic lateral flow test strip for

Journal of Immunological Methods 312 (2006) 27–33 /locate/jimResearch paper Development of an immunochromatographic lateral flow test strip for detection of β-adrenergic agonist Clenbuterol residues G.P. Zhang a,b,?, X.N. Wang a,b,?, J.F. Yang b, Y.Y. Yang b, G.X. Xing b, Q.M. Li b, D. Zhao b, S.J. Chai b, J.Q. Guo b a College of Animal and Veterinary Sciences, Henan Institute of Science and Technology, Xinxiang 453003, PR China b Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China Received 2 August 2005; received in revised form 19 January 2006; accepted 6 February 2006 Available online 25 April 2006Abstract A rapid immunochromatographic lateral flow test strip was developed in a competitive format with the gold-conjugated monoclonal antibody to specifically determine the residues of Clenbuterol (CL), a β-adrenergic agonist. The test strip is made up of a sample pad, a conjugate reagent pad, a test membrane containing a control line and a test line, and an absorbent pad. CL standard samples of 0, 0.1, 0.3, 0.9, 2.7, 8.1 ng/ml in swine urine were determined by the test strip. It was shown that detection limit of the test strip was as low as 0.1 ng/ml of CL and that the half of maximal inhibition concentration (IC50) in relative optical density was calculated to be 1.78±0.17 ng/ml under an optical density scanner. The sensitivity by eye measurement was 1.0 ng/ml. It takes 10 min to accomplish a test. Parallel analysis of urine samples from pigs fed with CL showed comparable results obtained from the test strip and GC-MS. Therefore, the test strip is very useful as a screening method for quantitative, semi-quantitative or qualitative detection of CL residues in swine urine. ? 2006 Elsevier B.V. All rights reserved.Keywords: Clenbuterol; Test strip; Colloidal gold; Immunochromatography; Rapid testAbbreviations: CL, Clenbuterol; BSA, bovine serum albumin; OVA, ovalbumin; HPLC, high performance liquid c