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Development of an immunochromatographic lateral flow test strip for
Journal of Immunological Methods 312 (2006) 27–33
/locate/jimResearch paper
Development of an immunochromatographic lateral flow test strip for
detection of β-adrenergic agonist Clenbuterol residues
G.P. Zhang a,b,?, X.N. Wang a,b,?, J.F. Yang b, Y.Y. Yang b, G.X. Xing b, Q.M. Li b,
D. Zhao b, S.J. Chai b, J.Q. Guo b
a College of Animal and Veterinary Sciences, Henan Institute of Science and Technology, Xinxiang 453003, PR China
b Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China
Received 2 August 2005; received in revised form 19 January 2006; accepted 6 February 2006
Available online 25 April 2006Abstract
A rapid immunochromatographic lateral flow test strip was developed in a competitive format with the gold-conjugated
monoclonal antibody to specifically determine the residues of Clenbuterol (CL), a β-adrenergic agonist. The test strip is made up of
a sample pad, a conjugate reagent pad, a test membrane containing a control line and a test line, and an absorbent pad. CL standard
samples of 0, 0.1, 0.3, 0.9, 2.7, 8.1 ng/ml in swine urine were determined by the test strip. It was shown that detection limit of the
test strip was as low as 0.1 ng/ml of CL and that the half of maximal inhibition concentration (IC50) in relative optical density was
calculated to be 1.78±0.17 ng/ml under an optical density scanner. The sensitivity by eye measurement was 1.0 ng/ml. It takes
10 min to accomplish a test. Parallel analysis of urine samples from pigs fed with CL showed comparable results obtained from the
test strip and GC-MS. Therefore, the test strip is very useful as a screening method for quantitative, semi-quantitative or qualitative
detection of CL residues in swine urine.
? 2006 Elsevier B.V. All rights reserved.Keywords: Clenbuterol; Test strip; Colloidal gold; Immunochromatography; Rapid testAbbreviations: CL, Clenbuterol; BSA, bovine serum albumin;
OVA, ovalbumin; HPLC, high performance liquid c
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