Human IL-12(P70)ELISA Kit说明书.pdf

RayBio? Human IL-12 (P70) ELISA Kit User Manual (Revised Mar 1, 2012) RayBio? Human IL-12(P70) ELISA Kit Protocol (Cat#: ELH-IL12P70) RayBiotech, Inc. We Provide You With Excellent Protein Array System And Service Tel:(Toll Free)1-888-494-8555 or 770-729-2992; Fax:770-206-2393; Web: Email: info@ RayBiotech, Inc. RayBio? Human IL-12(P70) ELISA Kit Protocol TABLE OF CONTENTS I. Introduction……..……………………………….2 II. Reagents……………………………..…………..2 III. Storage.………………………………………….3 IV. Additional Materials Required…………………..3 V. Reagent Preparation……………………………..4 VI. Assay Procedure………………………………...6 VII. Assay Procedure Summary……………………...7 VIII. Calculation of Results A. Typical Data…..……………………….…….…..8 B. Sensitivity……………………………….….……8 C. Recovery…………………………..……………..8 D. Linearity………………………………….…..... .9 E. Reproducibility…………………………………..9 IX. Specificity………………………………………..9 X. References…………………………………...…10 XI. Troubleshooting Guide.……………….………..11 RayBio? Human IL-12(P70) ELISA Kit Protocol 1 I. INTRODUCTION IL-12 is a heterodimeric 70 kDa glycoprotein (IL-12-p70) consisting of a 40 kDa subunit. It is secreted by peripheral lymphocytes after induction and is produced mainly by B-cells and to a lesser extent by T-cells. The most powerful inducers of IL-12 are bacteria, bacterial products, and parasites. IL- 12 is produced after stimulation with Phorbol esters or Calcium ionophore by human B-lymphoblastoid cells. IL-12 activates NK-cells positive for CD56, and this activity is blocked by antibodies specific for TNF-alpha. IL-12 promotes specific allogenic CTL reactions. IL-12 synergizes also with anti- CD3 antibodies and with allogeneic stimulation in mixed lymphocyte cultures in inducing T-cell proliferation. The RayBio? Human IL-12(P70) ELISA (Enzyme-L