Illumina BeadArray平台介绍.pdf

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Illumina BeadArray平台介绍

1 Illumina? BeadArrayTM Platforms Sole-Source Specifications iScan TM System Performance Specifications Scanner resolution 0.53 micron true spatial resolution with dual-channel collection Laser excitation 532nm and 658nm Emission spectrum 550–600nm and 677–697nm Detection Hardware and iScan Reader Bench footprint 52cm W x 45cm H x 66cm D (20” W x 18” H x 26” D) Sample Preparation Samples can be prepared manually or with automation using industry-standard liquid-handling robotics Scan Times (approximate) BeadChip Minutes Human610-Quad 7 Human1M-Duo 14 iSelect? 0.5 HumanMethylation27 2 HumanWG-6 Gene Expression 2 MouseRef-8 Gene Expression 2 RatRef-12 Gene Expression 2 Common software interface Supported BeadStudio has the same architecture for all supported applications Integrated Controls Dashboards GT, GX, and Methylation Chromosomal Viewer GT, GX, and Methylation Genome Browser GT, GX, and Methylation Chromosomal Heat Map GT, GX, and Methylation Heat Map with clustering algorithm GT, GX, and Methylation Bookmarking for CN/LOH GT Software and Data Analysis Auto bookmarking GT 2 Performance Specifications Export to third-party program GT, GX, Methylation Normalization GT, GX, Methylation Differential analysis GX, Methylation DASL? analysis GX, Methylation Warranty and Support One-year warranty coverage includes: ? iScan Reader (one-year parts and labor warranty, including laser replacements) ? One annual preventive maintenance visit ? All parts, labor, and travel costs associated with any repair, routine maintenance, and mandatory upgrades ? All mandatory hardware and software upgrades to the system Analysis Formats Microarrays in Array of Arrays? format, allowing multi-sample processing: ? BeadChip—variable formats aligned to application with 1, 2, 4, 6, 8, 12, or 16 samples in parallel Automation Options Performance Specifications Supported array format BeadChip format only AutoLoader2

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