postprandial blood glucose.pdf

BioMed CentralNutrition Metabolism ssOpen AcceResearch Suppressive response of confections containing the extractive from leaves of Morus Alba on postprandial blood glucose and insulin in healthy human subjects Mariko Nakamura, Sadako Nakamura and Tsuneyuki Oku* Address: Graduate School of Human Health Science, Siebold University of Nagasaki, 1-1-1 Manabino, Nagayo, Nagasaki, 851-2195, Japan Email: Mariko Nakamura - marikon@sun.ac.jp; Sadako Nakamura - nacsac@sun.ac.jp; Tsuneyuki Oku* - okutsune@sun.ac.jp * Corresponding author Abstract Background: The first aim of this study was to clarify the effective ratio of extractive from leaves of Morus Alba (ELM) to sucrose so as to apply this knowledge to the preparation of confections that could effectively suppress the elevation of postprandial blood glucose and insulin. The second aim was to identify the efficacy of confections prepared with the optimally effective ratio determined from the first study, using healthy human subjects. Methods: Ten healthy females (22.3 years, BMI 21.4 kg/m2) participated in this within-subject, repeated measures study. For the first aim of this study, the test solutions containing 30 g of sucrose and 1.2 or 3.0 g of ELM were repeatedly and randomly given to each subject. To identify the practically suppressive effects on postprandial blood glucose and insulin, some confections with added ELM were prepared as follows: Mizu-yokan, 30 g of sucrose with the addition of 1.5 or 3.0 g ELM; Daifuku-mochi, 9.0 g of starch in addition to 30 g of sucrose and 1.5 or 3.0 g ELM; Chiffon- cake, 24 g of sucrose, starch, and 3.0 or 6.0 g of ELM, and were ingested by each subject. Blood and end-expiration were collected at selected periods after test food ingestion. Results: When 30 g of sucrose with 1.2 or 3.0 g of ELM were ingested by subjects, the elevations of postprandial blood glucose and insulin were effectively suppressed (p 0.01), and the most effective ratio of ELM to sucrose was evaluate