QIAGEN 试剂盒.pdf

QIAamp? DNA FFPE Tissue Handbook For purification of genomic DNA from formalin-fixed, paraffin-embedded tissues April 2010 Sample Assay Technologies 1063027-HB 28.04.2010 10:33 Uhr Seite 1 Trademarks: QIAGEN?, QIAamp?, MinElute?, Qproteome?, REPLI-g?, RNeasy? (QIAGEN Group). ? 2007 QIAGEN, all rights reserved. QIAGEN is a member of the Forest Stewardship Council (FSC). For the production of printed materials, including handbooks, QIAGEN has a policy to select suppliers that comply with FSC standards for printing processes and well-managed forests. 1063027-HB 28.04.2010 10:33 Uhr Seite 2 QIAamp DNA FFPE Tissue Handbook 04/2010 3 Contents Kit Contents 4 Storage 4 Quality Control 4 Product Warranty and Satisfaction Guarantee 5 Technical Assistance 5 Safety Information 6 Product Use Limitations 7 Introduction 8 Principle and procedure 8 Equipment and Reagents to Be Supplied by User 10 Important Notes 11 Starting material 11 Copurification of RNA 11 Eluting pure DNA 11 Handling of QIAamp MinElute columns 12 Preparation of buffers 13 Protocol ■ Isolation of Genomic DNA from FFPE Tissue Sections 14 Troubleshooting Guide 17 Appendix: Working with DNA 19 Ordering Information 20 1063027-HB 28.04.2010 10:33 Uhr Seite 3 Kit Contents QIAamp DNA FFPE Tissue Kit (50) Catalog no. 56404 Number of preps 50 QIAamp MinElute? Columns 50 Collection Tubes (2 ml) 3 x 50 Buffer ATL 10 ml Buffer AL* 12 ml Buffer AW1* (concentrate) 19 ml Buffer AW2? (concentrate) 13 ml Buffer ATE? 12 ml Proteinase K 1.25 ml Handbook 1 * Contains a guanidine salt. Not compatible with disinfectants containing bleach. See page 6 for safety information. ? Contains sodium azide as a preservative. Storage QIAamp MinElute columns should be stored at 2–8°C upon arrival and are stable under these conditions for at least one year after delivery. However, short-term storage of up to 4 weeks at room temperature (15–25°C) does not affect performance. All buffers can be stored at room temperature (15–25°C) an