Small Intestinal Mucositis in the Rat.pdf

? 20 05 L A N D ES B IO SC IE N CE . D O N O T D IS TR IB U TE . [Cancer Biology Therapy 5:1, 34-38, January 2006]; ?2005 Landes Bioscience 34 Cancer Biology Therapy 2006; Vol. 5 Issue 1 Julie M. Clarke 1,5 Nicole C. Pelton 2,3 Balazs H. Bajka 1,3 Gordon S. Howarth 1,2,3,4,5,6,7 Leanna C. Read 1,5 Ross N. Butler 2,3,4,5 1 Child Health Research Institute; 2 Centre for Paediatric and Adolescent Gastroenterology, Children, Youth and Women’s Health Service; Departments of 3 Physiology and 4 Paediatrics; University of Adelaide 5 Cooperative Research Centre for Tissue Growth and Repair; 6 School of Pharmaceutical, Molecular and Biomedical Sciences; University of South Australia 7 School of Biological Sciences; Flinders University; Adelaide, South Australia, Australia *Correspondence to: Ross N. Butler; Children, Youth and Women’s Health Service; Centre for Paediatric and Adolescent Gastroenterology; 72 King William Road; North Adelaide, South Australia 5006 Australia; Tel.: +61.8.8161.6805; Fax: +61.8.8161.6088; Email: ross.butler@.au Received 09/01/05; Accepted 10/12/05 Previously published online as a Cancer Biology Therapy E-publication: /journals/cbt/abstract.php?id=2235 KEY WORDS 13C-sucrose, breath test, sucrase, mucositis, methotrexate, folinate, rat ABBREVIATIONS DAR Dark Agouti rat HE Hematoxylin and eosin MPO Myeloperoxidase MTX Methotrexate SBT Sucrose breath test AUC Area under curve ACKNOWLEDGEMENTS The authors wish to thank Helen Bougesis and Ben Edwards for conducting the animal trials, Caroline Payne for biochemical analyses and Katie Inglis for assistance with the preparation of the manuscript. The Co-operative Research Centre for Tissue Growth and Repair and Nestec Ltd are also acknowledged and thanked for financial support of this study. Research Paper Use of the 13 C-Sucrose Breath Test to Assess Chemotherapy-Induced Small Intestinal Mucositis in the Rat ABSTRACT Mucositis is a debilitating side-effect of chemotherapy which affects the mucos