羟基酸辅助尿素包合法分离纯化亚油酸.docVIP

2015 届毕业生 毕业论文 题 目: 羟基酸辅助尿素包合法富集多不饱和脂肪酸  摘 要 本文中红花籽油经皂化、酸解获得混合脂肪酸。以95%乙醇为溶剂，利用苹果酸辅助尿素包合法分离、纯化红花籽油中的亚油酸。以亚油酸的收率和纯度作为参考依据，研究了尿素∕苹果酸（摩尔比）、尿素∕脂肪酸（质量比）、溶剂∕脂肪酸（v∕m）、包合温度、包合时间等因素对尿素包合法分离纯化亚油酸的影响。最后由正交试验确定较为合适的反应条件。 单因素实验结果表明：亚油酸的纯度随苹果酸用量的增加而降低，收率则升高，苹果酸/尿素为2:5时较为合适。尿素的用量对纯度影响较大，纯度随尿素用量的增加而升高，收率则下降，尿素/脂肪酸为2.5:1时较为合适。溶剂的用量增加时，纯度先增加后降低，收率一直上升，溶剂/尿素为9:1时较为合适。包合温度和包合时间对实验影响不大。 正交实验结果表明：影响尿素包合法分离纯化亚油酸最主要的因素为尿素的用量，其次为溶剂用量，温度的影响并不大。不同的因素对纯度和收率的影响程度不一样。考虑实际情况，反应条件为：尿素∕苹果酸（摩尔比）2.5/1、尿素∕脂肪酸（质量比）3/1，溶剂∕脂肪酸（v∕m）12/1，包合温度5℃，包合时间16h。在此条件下亚油酸的纯度达到%，收率达到%。 关键词： 红花籽油 尿素包合法 亚油酸 纯度 收率 Title Separation and Purification of Linoleic Acid from Safflower Seed Oil by Hydroxy acid assisted urea adduction method. Abstract In this paper, the mixed fatty acids were obtained by the acid hydrolysis of safflower seed oil. Using 95% ethanol as solvent, the linoleic acid of safflower seed oil was purified by the method of Malic acid assisted urea adduction .The yield and purity of linoleic acid as a reference to study the urea / malic acid (molar ratio), urea / fatty acid (mass ratio), solvent / fatty acid (V / M), temperature, time of urea adduction of legal separation effect of purified linoleic acid. Finally, to determine the optimum reaction conditions by the orthogonal experiment. The results of univariate tests show that: the purity of linoleic acid decreased with increasing the amount of malic acid, while the yield increased, malic acid/urea as 2:5 relatively appropriate. Influence on the amount of urea for purity, and the purity increases with an increase in amount of urea, while the yield declined, urea/fatty acids was 2.5:1 is more appropriate. The dosage of the solvent increases, the purity increased after the first is reduced, the yield has been rising, solvent/urea for 9:1 relatively appropriate. Inclusion temperature and closure time of the experiment. Orthogonal experiment results show that: most important factor that impact the linoleic acid separation and