(Reactive Violet 5R) decolorising native acclimatised bacterial consortia.pdf

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(Reactive Violet 5R) decolorising native acclimatised bacterial consortia.pdf

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(Reactive Violet 5R) decolorising native acclimatised bacterial consortia

Bioresource Technology 142 (2013) 436–444Contents lists available at SciVerse ScienceDirect Bioresource Technology journal homepage: www.elsevier .com/locate /bior techMolecular fingerprinting of bacterial communities in enriched azo dye (Reactive Violet 5R) decolorising native acclimatised bacterial consortia0960-8524/$ - see front matter 2013 Elsevier Ltd. All rights reserved. /10.1016/j.biortech.2013.05.057 ? Corresponding author. Tel.: +91 265 2794396; fax: +91 265 2792508. E-mail addresses: archanagayatri@, garchana@ (G. Archana).Jagat Rathod, G. Archana ? Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390 002, Gujarat, Indiah i g h l i g h t s Both pristine and contaminated niches lead to development of acclimatised consortia. Total 12 efficient RV5R decolorising acclimatised consortia were enriched in lab. Community profiling was done using ARDRA and DGGE analysis. Consortia were comprised of decolorising and non-decolorising members. Acclimatised consortium Gly was found to degrade RV5R.g r a p h i c a l a b s t r a c ta r t i c l e i n f o Article history: Received 12 April 2013 Received in revised form 11 May 2013 Accepted 15 May 2013 Available online 22 May 2013 Keywords: Azo dye decolorisation Acclimatised bacterial consortia Amplified ribosomal DNA restriction digestion analysis (ARDRA) Denaturing gradient gel electrophoresis (DGGE) Fourier transformed infrared spectroscopy (FTIR)a b s t r a c t Reactive Violet 5R (RV5R) decolorising acclimatised bacterial consortia were enriched from industrial effluent contaminated and pristine samples from Gujarat, India on several different media. Twelve accli- matised consortia were selected for the study which were able to decolorise 100 mg/L RV5R in 30 h under shaking or static conditions. Eubacterial diversity was studied by 16S rRNA gene based culture-indepen- dent methods, using HaeIII and Hinf1 enzymes for ARDRA and V3