A Rhabdovirus Isolated from Grass Carp.pdf

Archives of Virology 4L8, 181--185 (1975) ? by Springer-Verlag 1975 A Rhabdovirus Isolated from Grass Carp (Ctenophargngodon idella Val.) Brief Report By W. A ~ E Institute of Zoology and I-IydrobiologTg, Ludwig Maximilians University of Munich, Federal Republic of Germany With 1 Figure Accepted February 27, 1975 During recent years the production of East-Asiatic cyprinids especially the grass carp (Ctenopharyngodon idella Val.) has steadily increased in Europe. The grass carp is mainly kept together with the common carp (Cyprinus carpio L.). Carp fish farmers are faced with two serious infectious diseases: the infectious dropsy (IDC) and the swim bladder inflammation (S]3I). Both diseases have been diagnosed in fish species other than carp (2, 13). An outbreak of a disease among grass carp similar to IDC has been observed and the authors pointed out that although the symptoms clearly indicated IDC, common carps living in the same pond were not infected (12). Since l~habdovirus earpio (RVC) causes spring viremia of carp (SVC) (5) and a similar if n.ot identical virus is associated with 8131 (1, 3, 4), the importance of infectious virus diseases in carp hatcheries is emphasized. In April 197zi a moribund three year old grass carp with ventral hemorrhagic inflammation, bleedings in the scale bases, necrotic fins, inflammation of the stomach, serous liquid in the abdomen, swoilen spleen, pale liver with swollen capillary vessels, opaque internM wall of the swim bladder with petechial he- morrhages and widened blood vessels was sent to this laboratory. Tissue samples from kidney, liver, spleen and intestine were collected asep- tically, homogenized, diluted 1 : 20 in P]3S and centrifuged at 2000 g for 20 minutes (4 ° C). The supernatant was then filtered through a 450 nm membrane filter and the filtrate inoculated in amounts of 0,5 ml onto FgM-(fathead minnow)-eell cultures (CCL-42) grown in Leighton tubes using Eagles MEM (EMEM) supple