APPENDIX GICCVAM RECOMMENDED HET-CAM TEST METHOD PROTOCOL.pdf

APPENDIX GICCVAM RECOMMENDED HET-CAM TEST METHOD PROTOCOL.pdf

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APPENDIX GICCVAM RECOMMENDED HET-CAM TEST METHOD PROTOCOL

ICCVAM Test Method Evaluation Report: Appendix G November 2006 APPENDIX G ICCVAM RECOMMENDED HET-CAM TEST METHOD PROTOCOL G-1 ICCVAM Test Method Evaluation Report: Appendix G November 2006 [This Page Intentionally Left Blank] G-2 ICCVAM Test Method Evaluation Report: Appendix G November 2006 ICCVAM Recommended Protocol for Future Studies Using the Hens Egg Test- Chorioallantoic Membrane (HET-CAM) Test Method PREFACE The protocol was adapted from the protocol previously described by Spielmann and Liebsch (INVITTOX 1992). Examples of use of the protocol can be found in Luepke (1985), Balls et al. (1995), Gilleron et al. (1996, 1997), and Spielmann et al. (1996). Future studies using the HET-CAM test method could include further characterization of the usefulness or limitations of the HET-CAM test method in a weight of evidence approach for regulatory decision making. Users should be aware that the proposed test method protocol could be revised based on any additional optimization and/or validation studies that are conducted in the future. ICCVAM recommends that test method users consult the ICCVAM/NICEATM website (/) to ensure use of the most current test method protocol. G-3 ICCVAM Test Method Evaluation Report: Appendix G November 2006 1.0 PURPOSE AND APPLICABILITY The purpose of this protocol to describe the components and procedures used to evaluate the potential

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