Aqueous two-phase extraction for protein recovery from corn extracts.pdf

下载文档

3
0
约5.41万字
约 13页
2017-04-09 发布于江苏
举报
版权申诉
保障服务

Aqueous two-phase extraction for protein recovery from corn extracts.pdf

1、本文档共13页，可阅读全部内容。
2、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

Aqueous two-phase extraction for protein recovery from corn extracts

Ai p p m f l s t s p t a A r ? K 1 e i p t n t n w s c 1 dJournal of Chromatography B, 845 (2007) 38–50 Aqueous two-phase extraction for protein recovery from corn extracts Zhengrong Gu, Charles E. Glatz ? Department of Chemical and Biological Engineering, 2114 Sweeney Hall, Iowa State University, Ames, IA 50011-2230, USA Received 27 April 2006; accepted 17 July 2006 Available online 22 August 2006 bstract Corn has been used as an expression host for several recombinant proteins with potential for large-scale production. Cost-effective downstream nitial recovery, separation and concentration remain a challenge. Aqueous two-phase (ATP) partitioning has been used to recover and concentrate roteins from fermentation broths and offers advantages for integration of those steps with biomass removal. To examine the applicability of ATP artitioning to recombinant protein purification from corn endosperm and germ, ATP system parameters including poly(ethylene glycol) (PEG) olecular weight (MW), phase-forming salt, tie line length (TLL), and pH were manipulated to control partitioning of extracted native proteins rom each fraction. Moderate PEG MW, reduction of phase ratio, and added NaCl effected complete recovery of the hydrophobic model protein ysozyme in the top phase with ca. 5× enrichment and illustrates a favorable match of recombinant protein characteristics, expression host, and eparation method. Furthermore, integration of protein extraction with the partitioning reduced the load of contaminating host proteins relative to he more traditional separate steps of extraction followed by partitioning. Performance of the integrated partitioning was hindered by endosperm olids loading, whereas for germ, which has ca. 35× higher aqueous soluble protein, the limit was protein solubility. For more hydrophilic model roteins (the model being cytochrome c), effective separation required further reduction of PEG MW to effect more partitioning of host proteins to he top phase and e