BAB2007-Bacillus minimum genome factory-effective utilization of microbial genome information.pdf

BAB2007-Bacillus minimum genome factory-effective utilization of microbial genome information.pdf

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BAB2007-Bacillus minimum genome factory-effective utilization of microbial genome information

Biotechnol. Appl. Biochem. (2007) 46, 169–178 (Printed in Great Britain) doi:10.1042/B169 MINIREVIEW Bacillus minimum genome factory: effective utilization of microbial genome information Katsutoshi Ara*1, Katsuya Ozaki*, Kouji Nakamura?, Kunio Yamane?, Junichi Sekiguchi? and Naotake Ogasawara§ *Biological Science Laboratories, Kao Co., Ltd., 2606 Akabane, Ichikaimachi, Haga, Tochigi 321-3497, Japan, ?Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8572, Japan, ?Faculty of Textile Science and Technology, University of Shinshu, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan, and §Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan In 1997, the complete genomic DNA sequence of Bacillus subtilis (4.2 Mbp) was determined and 4100 genes were identified [Kunst, Ogasawara, Moszer, Albertini, Alloni, Azevedo, Bertero, Bessieres, Bolotin, Borchert, S. et al. (1997) Nature 90, 249–256]. In addition, B. subtilis, which shows an excellent ability to secrete proteins (enzymes) and antibiotics in large quantities outside the cell, plays an important role in industrial and medical fields. It is necessary to clarify the genes involved in the production of compounds by understanding the network of these 4100 genes and the proceeding analysis of genes of unknown functions. In promoting such a study, it is expected that the regul- atory system of B. subtilis can be simplified by the creation of a Bacillus strain with a reduced genome by discriminating genes unnecessary for the production of proteins from essential genes, and deleting as many of these unnecessary genes as possible, which may help to understand this complex network of genes. We have previously distinguished essential and non-essential genes by evaluating the growth and enzyme-producing properties of strains of B. subtilis in which about 3000 genes (except 271 essential gene

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