Beckman流式细胞仪-GALLIOS使用说明书.pdf

Gallios TM Quick Reference Purpose: The purpose of this Quick Reference is to provide a simple step by step outline of the information needed to perform various tasks on the system. We begin with basic tasks like starting up or shutting down the system and then proceed to more involved software tasks. It is organized according to the modules in the Gallios Flow Cytometer Training Modules. Major Headings (in blue) are the major groups of tasks. These headings may be further subdivided into subsections of the major task and appear in a logical sequence. For example, the Major task of creating a protocol is subdivided into the subsections: Select Parameters, Create Plots, Instrument Settings, Create Regions, Gate, Analyze, and Save Protocol. A list of the major tasks appears below in the Table of Contents. Table of Contents Startups P. 3 Shutdown P. 4 Creating a Protocol P. 5 Create a FlowPAGE P. 7 AutoSetup P. 8 Acquisition Manager (Panels) P. 9 Database P. 11 Offline Analysis P. 15 System Setup P. 19 PN A75199AA (May, 2009) 1 of 20 PN A75199AA (May, 2009) 2 of 20 Startups Check external Sheath tank, fill the Cleaning agent tank and Empty the Waste tank Power the Computer and Monitor On Enter a Windows password if any and select OK Double click icon. Select User name Enter User password and select Next Select Connect (to run samples) or Work Offline . Check gages System Pressure 30 Psi, Vac 17 in (Cytometer only) Commonly Used Toolbars: Save LMD Print Copy Undo PRISM Histogram Rectangle Elliptical PRISM Multilinear Gates Color Publish File Plot Plot Divider 2 region Blend Open LMD Save Cut Paste Redo Dot Density Polygon Quadrant Contour Linear PRISM Color Menus Workspace Toolbars Acquisition Manager Resource Explorer Plot Tabs Focus object Worklist file tab Panel file tab Protocols file tab Listmode